Tools - Reference - Detail

Reference Detail

Reference
Author		Lee YJ,Kim BG,Chong Y,Lim Y,Ahn JH
Title		Cation dependent O-methyltransferases from rice.
Abstract: Two lower molecular mass OMT genes (ROMT-15 and -17) were cloned from rice and expressed in Escherichia coli as glutathione S-transferase fusion proteins. ROMT-15 and -17 metabolized caffeoyl-CoA, flavones and flavonols containing two vicinal hydroxyl groups, although they exhibited different substrate specificities. The position of methylation in both luteolin and quercetin was determined to be the 3' hydroxyl group and myricetin and tricetin were methylated not only at 3' but also at 5' hydroxyl groups. ROMT-15 and -17 are cation-dependent and mutation of the predicted metal binding sites resulted in the loss of the enzyme activity, indicating that the metal ion has a critical role in the enzymatic methylation.
Journal		Planta.
Country		South Korea
Volume		227
Pages		641-7
Year		2008
PubMed ID		17943312
PubMed Central ID		-
DOI		10.1007/s00425-007-0646-4
URL		-
Relation
Gene		_ _ _
INSD		-
Strain	Wild Core Collection	-
	Induced Mutation Lines(NIG Collection)	-
	Sterile Seed Strain	-
	Lethal Embryo Mutantion Strain	-
	Stages in Each Organ - Muant Lines (Gene)	-
	Cultivated Varieties(NIG Collection)	-
Stages in Each Organ		-