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Reference Detail
| Reference | ||
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| Author | Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK | |
| Title | OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints. | |
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Abstract: Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderophore uptake and/or metal-nicotianamine translocation. Among the 18 YSL genes in rice (OsYSLs), OsYSL18 is predicted to encode a polypeptide of 679 amino acids containing 13 putative transmembrane domains. An OsYSL18-green fluorescent protein (GFP) fusion was localized to the plasma membrane when transiently expressed in onion epidermal cells. Electrophysiological measurements using Xenopus laevis oocytes showed that OsYSL18 transports iron(III)-deoxymugineic acid, but not iron(II)-nicotianamine, zinc(II)-deoxymugineic acid, or zinc(II)-nicotianamine. Reverse transcriptase PCR analysis revealed more OsYSL18 transcripts in flowers than in shoots or roots. OsYSL18 promoter-beta-glucuronidase (GUS) analysis revealed that OsYSL18 was expressed in reproductive organs including the pollen tube. In vegetative organs, OsYSL18 was specifically expressed in lamina joints, the inner cortex of crown roots, and phloem parenchyma and companion cells at the basal part of every leaf sheath. These results suggest that OsYSL18 is an iron-phytosiderophore transporter involved in the translocation of iron in reproductive organs and phloem in joints. |
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| Journal | Plant Mol. Biol. | |
| Country | Japan | |
| Volume | 70 | |
| Pages | 681-92 | |
| Year | 2009 | |
| PubMed ID | 19468840 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s11103-009-9500-3 | |
| URL | - | |
| Relation | ||
| Gene | YSL1 YSL10 YSL11 YSL12 YSL13 YSL14 YSL15 YSL16 YSL17 YSL18 YSL2 YSL3 YSL4 YSL5 YSL6 YSL7 YSL8 YSL9 | |
| INSD | AB190926 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
