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Author Narayanan NN, Vasconcelos MW, Grusak MA.
Title Expression profiling of Oryza sativa metal homeostasis genes in different rice cultivars using a cDNA macroarray.
Abstract:
Rice is an important food crop, but it is a poor source of essential micronutrients such as iron and zinc. In order to improve the metal ion content of rice grains through breeding or biotechnology, more information is needed on the molecular players that help mobilize metals from leaves to developing seeds. To profile several genes simultaneously, a cDNA macroarray was developed using 36 metal-related genes from rice, including ZIPs, NRAMPs, and YSLs (coding for known or potential metal transporters), as well as NAS, FER, FRO, NAAT, FDH, GSTU, and PDR (involved in metal homeostasis). Because flag leaves are the major source of phloem-delivered photoassimilates and remobilized metals for developing seeds, we analyzed the expression of these metal-related genes in flag and non-flag leaves of four different rice cultivars (Cocodrie, Taipei 309, IR58, and IR68144) during the period of mid-grain fill. Genes (24 of 36) exhibited low to non-detectable signals in the macroarray, while 12 genes (OsIRT1, OsZIP1, OsZIP5, OsZIP8, OsYSL5, OsYSL6, OsYSL7, OsYSL8, OsYSL18, OsNRAMP2, OsNRAMP4 and OsNRAMP7) were found to be highly expressed in both flag and non-flag leaves of all four cultivars. Additional expression analysis using semi-quantitative or quantitative PCR provided results that were generally consistent with the macroarray, but semi-quantitative PCR confirmed that OsFDH, OsFER1, OsNAAT, OsNAS1, OsPDR9, OsYSL12, OsYSL13, OsZIP7, and OsZIP10 were also expressed in leaves. This specialized macroarray has provided a short list of potential candidate genes, expressed in leaves, which might contribute to the process of metal transport to distant sinks, such as seeds.
Journal Plant Physiol. Biochem.
Country USA
Volume 45(5)
Pages 277-86.
Year 2007
PubMed ID 17468002
PubMed Central ID -
DOI 10.1016/j.plaphy.2007.03.021
URL -
Relation
Gene FDH FER1 FER2 FRO1 FRO2 IRT1 LCD1 NAAT NAS1 NAS2 NRAMP1 NRAMP2 NRAMP4 NRAMP6 NRAMP7 PDR9 YSL12 YSL13 YSL16 YSL17 YSL18 YSL19 YSL2 YSL5 YSL6 YSL7 YSL8 ZIP1 ZIP2 ZIP3 ZIP5 ZIP6 ZIP7
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase