Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Cheng,X., Liu,G., Ye,G., Wang,H., Shen,X., Wu,K., Xie,J. and Altosaar,I. | |
| Title | Screening and cloning of antimicrobial DNA sequences using a vital staining method | |
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Abstract: A novel method for cloning of genes coding for cytotoxic molecules based on a cell viability assay is described. The working hypothesis is that expression of DNA sequences coding for cytotoxic molecules in bacterial cells will lead to cell death or impairment, and the isolation of the impaired or dead cells could lead to identification of DNA sequences responsible for debilitating the host cells. We verified this concept by isolating the well known antimicrobial Puroindoline b gene in Escherichia coli cells. We further demonstrated the feasibility to use this approach for isolating DNA encoding for antimicrobials from cDNA expression libraries. Sequence analysis and bioassay indicated that the isolated clones encoded previously characterized antimicrobial proteins (AMPs), proteins not previously characterized as AMPs, as well as novel antimicrobial peptides. In addition, clones harboring ribosomal protein encoding cDNA were also identified. Therefore, this method could also be used to identify host genes important in maintaining bacterial cell viability. |
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| Journal | Gene | |
| Country | ||
| Volume | 430(1-2) | |
| Pages | 132-139 | |
| Year | 2009 | |
| PubMed ID | 19027836 | |
| PubMed Central ID | - | |
| DOI | 10.1016/j.gene.2008.10.017 | |
| URL | - | |
| Relation | ||
| Gene | DHN1 GER3 LEA16 LEA3-1 PRPL3 RAB16B TOND1 _ | |
| INSD | EF444535 EF444536 EF444528 EF444529 EF444530 EF444531 EF444532 EF444533 EF444534 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
