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著者 | Wong HL,Pinontoan R,Hayashi K,Tabata R,Yaeno T,Hasegawa K,Kojima C,Yoshioka H,Iba K,Kawasaki T,Shimamoto K | |
タイトル | Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. | |
Abstract: Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein NADPH oxidase complex of phagocytes, in plants, only the homologs of the catalytic subunit gp91phox and the cytosolic regulator small GTPase Rac are found. Plant homologs of the gp91phox subunit are known as Rboh (for respiratory burst oxidase homolog). Although numerous Rboh have been isolated in plants, the regulation of enzymatic activity remains unknown. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+ binding EF-hand motifs. Previously, we ascertained that a small GTPase Rac (Os Rac1) enhanced pathogen-associated molecular pattern-induced ROS production and resistance to pathogens in rice (Oryza sativa). In this study, using yeast two-hybrid assay, we found that interaction between Rac GTPases and the N-terminal extension is ubiquitous and that a substantial part of the N-terminal region of Rboh, including the two EF-hand motifs, is required for the interaction. The direct Rac-Rboh interaction was supported by further studies using in vitro pull-down assay, a nuclear magnetic resonance titration experiment, and in vivo fluorescence resonance energy transfer (FRET) microscopy. The FRET analysis also suggests that cytosolic Ca2+ concentration may regulate Rac-Rboh interaction in a dynamic manner. Furthermore, transient coexpression of Os Rac1 and rbohB enhanced ROS production in Nicotiana benthamiana, suggesting that direct Rac-Rboh interaction may activate NADPH oxidase activity in plants. Taken together, the results suggest that cytosolic Ca2+ concentration may modulate NADPH oxidase activity by regulating the interaction between Rac GTPase and Rboh. |
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掲載誌 | Plant Cell | |
Country | Japan | |
刊数 | 19 | |
ページ | 4022-34 | |
発刊年 | 2007 | |
PubMed ID | 18156215 | |
PubMed Central ID | - | |
DOI | 10.1105/tpc.107.055624 | |
URL | - | |
関連情報 | ||
遺伝子 | RAC1 RAC2 RAC3 RAC4 RAC5 RAC6 RAC7 RBOHA RBOHB RBOHC RBOHD RBOHE RBOHF RBOHG RBOHH RBOHI | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |