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Reference Detail
| Reference | ||
|---|---|---|
| Author | Shimono M,Sugano S,Nakayama A,Jiang CJ,Ono K,Toki S,Takatsuji H | |
| Title | Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance. | |
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Abstract: Benzothiadiazole (BTH) is a so-called plant activator and protects plants from diseases by activating the salicylic acid (SA) signaling pathway. By microarray screening, we identified BTH- and SA-inducible WRKY transcription factor (TF) genes that were upregulated within 3 h after BTH treatment. Overexpression of one of them, WRKY45, in rice (Oryza sativa) markedly enhanced resistance to rice blast fungus. RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease, indicating that it is essential for BTH-induced defense responses. In a transient expression system, WRKY45 activated reporter gene transcription through W-boxes. Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1, a rice ortholog of Arabidopsis thaliana NPR1, which distinguishes WRKY45 from known Arabidopsis WRKY TFs. Two defense-related genes, encoding a glutathione S-transferase and a cytochrome P450, were found to be regulated downstream of WRKY45 but were not regulated by NH1, consistent with the apparent independence of the WRKY45- and NH1-dependent pathways. Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions, suggesting that some environmental factor(s) acts downstream of WRKY45 transcription. We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice, an importance food resource worldwide. |
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| Journal | Plant Cell | |
| Country | Japan | |
| Volume | 19 | |
| Pages | 2064-76 | |
| Year | 2007 | |
| PubMed ID | 17601827 | |
| PubMed Central ID | - | |
| DOI | 10.1105/tpc.106.046250 | |
| URL | - | |
| Relation | ||
| Gene | AROMA1 CYP709C9 GSTU4 LOX11 NH1 PR10A PR1A PR1B SGT WRKY45 WRKY62 WRKY76 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
