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Author Gu,Z., Ma,B., Jiang,Y., Chen,Z., Su,X. and Zhang,H.
Title Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses
Abstract:
Calcium plays a crucial role as a second messenger in mediating various defense responses under environmental stresses. Calcineurin B-like (CBL) proteins have been implicated as important Ca2+ sensors in plant-specific calcium signaling. Based on the similarity of sequence, ten CBL genes were identified by searching the rice japonica genome database, which were randomly distribute on chromosomes 1, 2, 3, 5, 10 and 12. By semi-quantitative RT-PCR approach the expression pattern of each gene was detected in various organs at the adult stage and seedlings treated with NaCl, PEG and cold stresses or exogenous ABA. The results showed that the induction of each rice CBL gene was not only responsive to different stress conditions, but also organ specific. In vivo targeting experiment revealed that OsCBL8 localized to the plasma membrane, which was consistent with OsCBL4 and SOS3 previously reported. To elucidate the putative function of OsCBL8 gene, transgenic rice plants overexpressing OsCBL8 gene were generated by the Agrobacterium-mediated approach. The OsCBL8 transgenic rice seedlings showed more tolerance to salt stress than non-transgenic seedlings.
Journal Gene
Country China
Volume 415(1-2)
Pages 1-12
Year 2008
PubMed ID 18395997
PubMed Central ID -
DOI 10.1016/j.gene.2008.02.011
URL -
Relation
Gene CBL1 CBL10 CBL2 CBL3 CBL5 CBL6 CBL7 CBL8 CBL9 SOS3
INSD DQ201204 DQ201195 DQ201196 DQ201197 DQ201198 DQ201199 DQ201200 DQ201201 DQ201202 DQ201203
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase