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| 著者 | Nishi A, Nakamura Y, Tanaka N, Satoh H | |
| タイトル | Biochemical and genetic analysis of the effects of amylose-extender mutation in rice endosperm | |
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Abstract: Biochemical analysis of amylose-extender (ae) mutant of rice (Oryza sativa) revealed that the mutation in the gene for starch-branching enzyme IIb (BEIIb) specifically altered the structure of amylopectin in the endosperm by reducing short chains with degree of polymerization of 17 or less, with the greatest decrease in chains with degree of polymerization of 8 to 12. The extent of such change was correlated with the gelatinization properties of the starch granules, as determined in terms of solubility in urea solution. The ae mutation caused a dramatic reduction in the activity of BEIIb. The activity of soluble starch synthase I (SSI) in the ae mutant was significantly lower than in the wild type, suggesting that the mutation had a pleiotropic effect on the SSI activity. In contrast, the activities of BEI, BEIIa, ADP-Glc pyrophosphorylase, isoamylase, isoamylase, pullulanase, and Suc synthase were not affected by the mutation. Therefore, it is stressed that the function of BEIIb cannot be complemented by BEIIa and BEI. These results strongly suggest that BEIIb plays a specific role in the transfer of short chains, which might then be extended by SS to form the A and B(1) chains of amylopectin cluster in rice endosperm. |
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| 掲載誌 | Plant Physiol. | |
| Country | Japan | |
| 刊数 | 127(2) | |
| ページ | 459-72 | |
| 発刊年 | 2001 | |
| PubMed ID | 11598221 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.127.2.459 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AE SBE1 SBE3 SBE4 SSI | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
