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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zeng L, Yin Z, Chen J, Leung H, Wang GL. | |
| Title | Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome. | |
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Abstract: The rice lesion mimic mutant spl11 was previously found to confer broad-spectrum disease resistance to both Magnaporthe grisea and Xanthomonas oryzae pv. oryzae. To better understand the molecular basis underlying cell death and disease resistance in rice, a map-based cloning strategy has been employed to isolate Spl11. Five Spl11-linked RAPD markers were developed and four of them were mapped to rice chromosome 12. A high-resolution genetic map was developed using a segregating population consisting of 1138 lesion mimic individuals. Recombination suppression was observed in the vicinity of Spl11. Three molecular markers tightly linked to Spl11 were identified and used to screen a BAC library. A contig spanning the Spl11 locus was constructed and physical mapping delimited Spl11 to a 160-kb DNA segment within a single BAC clone. These results provide the essential information for the final isolation of this important gene in the rice defense pathway. |
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| Journal | Molecular Genetics & Genomics (Molecular General Genetics up to 2001) | |
| Country | USA | |
| Volume | 268(2) | |
| Pages | 253-261 | |
| Year | 2002 | |
| PubMed ID | 12395200 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00438-002-0743-9 | |
| URL | - | |
| Relation | ||
| Gene | SPL11 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
