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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu G, Wang L, Zhou Z, Leung H, Wang GL, He C. | |
| Title | Physical mapping of a rice lesion mimic gene, Spl1 , to a 70-kb segment of rice chromosome 12. | |
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Abstract: The rice lesion mimic mutant spotted leaf 1 ( spl1) was first identified in the rice ( Oryza sativa) cultivar Asahi in 1965. This mutant displayed spontaneous disease-like lesions in the absence of any pathogen, and was found to confer resistance to multiple isolates of rice blast. We employed a map-based cloning strategy to localize the Spl1 gene. A total of ten cleaved amplified polymorphic sequence (CAPS) markers linked to the Spl1 gene were identified and mapped to an 8.5-cM region on chromosome 12. A high-resolution genetic map was developed using these ten CAPS markers and a segregating population consisting of 3202 individuals. A BAC contig containing four BAC clones was constructed, and Spl1 was localized to a 423-kb region. Seven spl1 mutants were obtained from the IR64 deletion mutant collection, and molecular analysis using these mutants delimited the Spl1 gene to a 70-kb interval, covered by two BAC clones. These results provide the basis for cloning this gene, which is involved in cell death and disease resistance in rice. |
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| Journal | Molecular Genetics & Genomics (Molecular General Genetics up to 2001) | |
| Country | China | |
| Volume | 272(2) | |
| Pages | 108-115 | |
| Year | 2004 | |
| PubMed ID | 15258851 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00438-004-1040-6 | |
| URL | - | |
| Relation | ||
| Gene | SPL1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
