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| 文献 | ||
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| 著者 | Nishimura A, Ashikari M, Lin S, Takashi T, Angeles ER, Yamamoto T, Matsuoka M. | |
| タイトル | Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems. | |
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Abstract: Regeneration of plant organs is often the essential step in genetic transformation; however, the regeneration ability of a plant varies depending on the genetic background. By conventional crosses of low-regeneration rice strain Koshihikari with high-regeneration rice strain Kasalath, we identified some quantitative trait loci, which control the regeneration ability in rice. Using a map-based cloning strategy, we isolated a main quantitative trait loci gene encoding ferredoxin-nitrite reductase (NiR) that determines regeneration ability in rice. Molecular analyses revealed that the poor regeneration ability of Koshihikari is caused by lower expression than in Kasalath and the specific activity of NiR. Using the NiR gene as a selection marker, we succeeded in selectively transforming a foreign gene into rice without exogenous marker genes. Our results demonstrate that nitrate assimilation is an important process in rice regeneration and also provide an additional selectable marker for rice transformation. |
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| 掲載誌 | Proc. Natl. Acad. Sci. U.S.A. | |
| Country | ||
| 刊数 | 102(33) | |
| ページ | 11940-11944 | |
| 発刊年 | 2005 | |
| PubMed ID | 16091467 | |
| PubMed Central ID | - | |
| DOI | 10.1073/pnas.0504220102 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | PSR1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
