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Reference Detail
| Reference | ||
|---|---|---|
| Author | Tanida I, Kim JK, Wu R. | |
| Title | Functional dissection of a rice high-pI alpha-amylase gene promoter. | |
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Abstract: Deletion analysis has previously shown that a 260 bp fragment, located between positions -230 and +29 of the 5' end of a rice high-pI alpha--amylase gene, OSamy-c, is required for gibberellic acid (GA3)-dependent transcriptional activation. We have since established a quantitative transient assay based on expression of a luciferase reporter gene in rice aleurone cells and continued to characterize the OSamy-c promoter for GA3-dependent regulatory sequences. Using this method, we have shown that the DNA sequence between -158 and -46 (sequence I) is sufficient to confer GA3-responsive activation on OSamy-c. We have also shown that this sequence is capable of directing GA3-dependent expression from a heterologous minimal promoter. Our results also showed that sequence I confers GA3 regulatory control in an orientation-dependent manner and interacts with two further upstream DNA sequences, II and III, in a combination which mildly enhances the level of the GA3 response exhibited by sequence I. Thus, we propose that sequence I confers the fundamental GA3-responsive character on OSamy-c, and that regulatory proteins that bind sequences II and III interact with each other and with regulatory proteins that bind sequence I, effectively to modulate the GA3 response. |
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| Journal | Molecular Genetics & Genomics (Molecular General Genetics up to 2001) | |
| Country | ||
| Volume | 244(2) | |
| Pages | 127-134 | |
| Year | 1994 | |
| PubMed ID | 8052231 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | AMY1B | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
