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著者 | Wong HL, Sakamoto T, Kawasaki T, Umemura K, Shimamoto K. | |
タイトル | Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice. | |
Abstract: Metallothioneins are small, ubiquitous Cys-rich proteins known to be involved in reactive oxygen species (ROS) scavenging and metal homeostasis. We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors. Transgenic plants overexpressing OsMT2b showed increased susceptibility to bacterial blight and blast fungus. OsMT2b-overexpressing cells showed reduced elicitor-induced hydrogen peroxide production. In contrast, homozygous OsMT2b::Tos17-inserted mutant and OsMT2b-RNAi-silenced transgenic cells showed significantly higher elicitor-induced hydrogen peroxide production than the wild-type cells. In vitro assay showed that recombinant OsMT2b protein possessed superoxide- and hydroxyl radical-scavenging activities. Taken together, these results showed that OsMT2b is an ROS scavenger and its expression is down-regulated by OsRac1, thus potentiating ROS, which function as signals in resistance response. The results suggest that OsRac1 plays a dual role as an inducer of ROS production and a suppressor of ROS scavenging. |
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掲載誌 | Plant Physiol. | |
Country | Japan | |
刊数 | 135(3) | |
ページ | 1447-1456 | |
発刊年 | 2004 | |
PubMed ID | 15220467 | |
PubMed Central ID | - | |
DOI | 10.1104/pp.103.036384 | |
URL | - | |
関連情報 | ||
遺伝子 | MT2A MT2B MTI1A MTI2B MTI3A MTI3B MTI4A MTI4C MTII1A RAC1 | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |