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Reference Detail
| Reference | ||
|---|---|---|
| Author | Wong HL, Sakamoto T, Kawasaki T, Umemura K, Shimamoto K. | |
| Title | Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice. | |
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Abstract: Metallothioneins are small, ubiquitous Cys-rich proteins known to be involved in reactive oxygen species (ROS) scavenging and metal homeostasis. We found that the expression of a metallothionein gene (OsMT2b) was synergically down-regulated by OsRac1 and rice (Oryza sativa) blast-derived elicitors. Transgenic plants overexpressing OsMT2b showed increased susceptibility to bacterial blight and blast fungus. OsMT2b-overexpressing cells showed reduced elicitor-induced hydrogen peroxide production. In contrast, homozygous OsMT2b::Tos17-inserted mutant and OsMT2b-RNAi-silenced transgenic cells showed significantly higher elicitor-induced hydrogen peroxide production than the wild-type cells. In vitro assay showed that recombinant OsMT2b protein possessed superoxide- and hydroxyl radical-scavenging activities. Taken together, these results showed that OsMT2b is an ROS scavenger and its expression is down-regulated by OsRac1, thus potentiating ROS, which function as signals in resistance response. The results suggest that OsRac1 plays a dual role as an inducer of ROS production and a suppressor of ROS scavenging. |
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| Journal | Plant Physiol. | |
| Country | Japan | |
| Volume | 135(3) | |
| Pages | 1447-1456 | |
| Year | 2004 | |
| PubMed ID | 15220467 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.103.036384 | |
| URL | - | |
| Relation | ||
| Gene | MT2A MT2B MTI1A MTI2B MTI3A MTI3B MTI4A MTI4C MTII1A RAC1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
