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Reference Detail
| Reference | ||
|---|---|---|
| Author | Hirose,N., Makita,N., Kojima,M., Kamada-Nobusada,T. and Sakakibara,H. | |
| Title | Overexpression of a type-a response regulator alters rice morphology and cytokinin metabolism. | |
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Abstract: Genome-wide analyses of rice (Oryza sativa L.) cytokinin (CK)-responsive genes using the Affymetrix GeneChip(R) rice genome array were conducted to define the spectrum of genes subject to regulation by CK in monocotyledonous plants. Application of trans-zeatin modulated the expression of a wide variety of genes including those involved in hormone signaling and metabolism, transcriptional regulation, macronutrient transport and protein synthesis. To understand further the function of CK in rice plants, we examined the effects of in planta manipulation of a putative CK signaling factor on morphology, CK metabolism and expression of CK-responsive genes. Overexpression of the CK-inducible type-A response regulator OsRR6 abolished shoot regeneration, suggesting that OsRR6 acts as a negative regulator of CK signaling. Transgenic lines overexpressing OsRR6 (OsRR6-ox) had dwarf phenotypes with poorly developed root systems and panicles. Increased content of trans-zeatin-type CKs in OsRR6-ox lines indicates that homeostatic control of CK levels is regulated by OsRR6 signaling. Expression of genes encoding CK oxidase/dehydrogenase decreased in OsRR6-ox plants, possibly accounting for elevated CK levels in transgenic lines. Expression of a number of stress response genes was also altered in OsRR6-ox plants. |
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| Journal | Plant Cell Physiol. | |
| Country | Japan | |
| Volume | 48(3) | |
| Pages | 523-539 | |
| Year | 2007 | |
| PubMed ID | 17293362 | |
| PubMed Central ID | - | |
| DOI | 10.1093/pcp/pcm022 | |
| URL | - | |
| Relation | ||
| Gene | CKX11 CKX4 CKX5 GA2OX3 GN1A PRL5 RR1 RR10 RR11 RR12 RR13 RR2 RR3 RR4 RR5 RR6 RR7 RR8 TMK WRKY71 | |
| INSD | AB249653 AB249665 AB249664 AB249663 AB249662 AB249661 AB249660 AB249659 AB249658 AB249657 AB249656 AB249655 AB249654 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
