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著者 | Furukawa,T., Maekawa,M., Oki,T., Suda,I., Iida,S., Shimada,H., Takamure,I. and Kadowaki,K.I. | |
タイトル | The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. | |
Abstract: Different colors, such as purple, brown, red and white, occur in the pericarp of rice. Here, two genes affecting proanthocyanidin synthesis in red- and brown-colored rice were elucidated. Genetic segregation analysis suggested that the Rd and A loci are identical, and both encode dihydroflavonol-4-reductase (DFR). The introduction of the DFR gene into an Rcrd mutant resulted in red-colored rice, which was brown in the original mutant, demonstrating that the Rd locus encodes the DFR protein. Accumulation of proanthocyanidins was observed in the transformants by the introduction of the Rd gene into the rice Rcrd line. Protein blot analysis showed that the DFR gene was translated in seeds with alternative translation initiation. A search for the Rc gene, which encodes a transacting regulatory factor, was conducted using available DNA markers and the Rice Genome Automated Annotation System program. Three candidate genes were identified and cloned from a rice RcRd line and subsequently introduced into a rice rcrd line. Brown-colored seeds were obtained from transgenic plants by the introduction of a gene containing the basic helix-loop-helix (bHLH) motif, demonstrating that the Rc gene encodes a bHLH protein. Comparison of the Rc locus among rice accessions showed that a 14-bp deletion occurred only in the rc locus. |
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掲載誌 | Plant J. | |
Country | Japan | |
刊数 | 49(1) | |
ページ | 91-102 | |
発刊年 | 2007 | |
PubMed ID | 17163879 | |
PubMed Central ID | - | |
DOI | 10.1111/j.1365-313X.2006.02958.x | |
URL | - | |
関連情報 | ||
遺伝子 | RC RD | |
INSD | AB247503 AB250059 | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |