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Reference Detail
| Reference | ||
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| Author | Xie Z, Zhang ZL, Zou X, Huang J, Ruas P, Thompson D, Shen QJ. | |
| Title | Annotations and Functional Analyses of the Rice WRKY Gene Superfamily Reveal Positive and Negative Regulators of Abscisic Acid Signaling in Aleurone Cells | |
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Abstract: The WRKY proteins are a superfamily of regulators that control diverse developmental and physiological processes. This family was believed to be plant specific until the recent identification of WRKY genes in nonphotosynthetic eukaryotes. We have undertaken a comprehensive computational analysis of the rice (Oryza sativa) genomic sequences and predicted the structures of 81 OsWRKY genes, 48 of which are supported by full-length cDNA sequences. Eleven OsWRKY proteins contain two conserved WRKY domains, while the rest have only one. Phylogenetic analyses of the WRKY domain sequences provide support for the hypothesis that gene duplication of single- and two-domain WRKY genes, and loss of the WRKY domain, occurred in the evolutionary history of this gene family in rice. The phylogeny deduced from the WRKY domain peptide sequences is further supported by the position and phase of the intron in the regions encoding the WRKY domains. Analyses for chromosomal distributions reveal that 26% of the predicted OsWRKY genes are located on chromosome 1. Among the dozen genes tested, OsWRKY24, -51, -71, and -72 are induced by abscisic acid (ABA) in aleurone cells. Using a transient expression system, we have demonstrated that OsWRKY24 and -45 repress ABA induction of the HVA22 promoter-beta-glucuronidase construct, while OsWRKY72 and -77 synergistically interact with ABA to activate this reporter construct. This study provides a solid base for functional genomics studies of this important superfamily of regulatory genes in monocotyledonous plants and reveals a novel function for WRKY genes, i.e. mediating plant responses to ABA. |
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| Journal | Plant Physiol. | |
| Country | USA | |
| Volume | 137 (1) | |
| Pages | 176-189 | |
| Year | 2005 | |
| PubMed ID | 15618416 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | SGSD3 WRKY1 WRKY10 WRKY102 WRKY11 WRKY12 WRKY13 WRKY14 WRKY15 WRKY16 WRKY17 WRKY18 WRKY19 WRKY2 WRKY20 WRKY21 WRKY22 WRKY23 WRKY24 WRKY25 WRKY26 WRKY27 WRKY28 WRKY29 WRKY3 WRKY30 WRKY31 WRKY32 WRKY33 WRKY34 WRKY35 WRKY37 WRKY38 WRKY39 WRKY4 WRKY40 WRKY41 WRKY42 WRKY43 WRKY45 WRKY46 WRKY47 WRKY48 WRKY49 WRKY5 WRKY50 WRKY51 WRKY52 WRKY53 WRKY54 WRKY55 WRKY56 WRKY57 WRKY58 WRKY6 WRKY60 WRKY61 WRKY62 WRKY64 WRKY65 WRKY66 WRKY67 WRKY68 WRKY69 WRKY7 WRKY70 WRKY71 WRKY72 WRKY73 WRKY74 WRKY75 WRKY76 WRKY77 WRKY8 WRKY87 WRKY9 WRKY90 | |
| INSD | BK005213 BK005214 BK005215 BK005027 BK005048 BK005053 BK005074 BK005075 BK005080 BK005212 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
