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Reference
Author Zhang,Z.L., Xie,Z., Zou,X., Casaretto,J., Ho,T.H. and Shen,Q.J.
Title A Rice WRKY Gene Encodes a Transcriptional Repressor of the Gibberellin Signaling Pathway in Aleurone Cells
Abstract:
The molecular mechanism by which GA regulates plant growth and development has been a subject of active research. Analyses of the rice (Oryza sativa) genomic sequences identified 77 WRKY genes, among which OsWRKY71 is highly expressed in aleurone cells. Transient expression of OsWRKY71 by particle bombardment specifically represses GA-induced Amy32b alpha-amylase promoter but not abscisic acid-induced HVA22 or HVA1 promoter activity in aleurone cells. Moreover, OsWRKY71 blocks the activation of the Amy32b promoter by the GA-inducible transcriptional activator OsGAMYB. Consistent with its role as a transcriptional repressor, OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally defined TGAC-containing W boxes of the Amy32b promoter in vitro. Mutation of the two W boxes prevents the binding of OsWRKY71 to the mutated promoter, and releases the suppression of the OsGAMYB-activated Amy32b expression by OsWRKY71, suggesting that OsWRKY71 blocks GA signaling by functionally interfering with OsGAMYB. Exogenous GA treatment decreases the steady-state mRNA level of OsWRKY71 and destabilizes the GFP:OsWRKY71 fusion protein. These findings suggest that OsWRKY71 encodes a transcriptional repressor of GA signaling in aleurone cells.
Journal Plant Physiol.
Country USA
Volume 134 (4)
Pages 1500-1513
Year 2004
PubMed ID 15047897
PubMed Central ID -
DOI 10.1104/pp.103.034967
URL -
Relation
Gene WRKY1 WRKY10 WRKY11 WRKY12 WRKY13 WRKY14 WRKY15 WRKY16 WRKY17 WRKY18 WRKY19 WRKY2 WRKY20 WRKY21 WRKY22 WRKY23 WRKY24 WRKY25 WRKY26 WRKY27 WRKY28 WRKY29 WRKY3 WRKY30 WRKY31 WRKY32 WRKY33 WRKY34 WRKY35 WRKY36 WRKY37 WRKY38 WRKY39 WRKY4 WRKY40 WRKY41 WRKY42 WRKY43 WRKY45 WRKY46 WRKY47 WRKY48 WRKY49 WRKY5 WRKY50 WRKY51 WRKY52 WRKY53 WRKY54 WRKY55 WRKY56 WRKY57 WRKY58 WRKY6 WRKY60 WRKY61 WRKY62 WRKY63 WRKY64 WRKY65 WRKY66 WRKY67 WRKY68 WRKY69 WRKY7 WRKY70 WRKY71 WRKY72 WRKY73 WRKY74 WRKY75 WRKY76 WRKY77 WRKY8 WRKY9
INSD BK005041 BK005042 BK005043 BK005044 BK005045 BK005046 BK005047 BK005048 BK005049 BK005050 BK005051 BK005052 BK005053 BK005054 BK005055 BK005056 BK005057 BK005058 BK005059 BK005060 BK005061 BK005062 BK005063 BK005064 BK005065 BK005066 BK005067 BK005068 BK005069 BK005070 BK005071 BK005072 BK005073 BK005074 BK005075 BK005076 BK005077 BK005078 BK005079 BK005080 BK005026 BK005025 BK005024 BK005023 BK005022 BK005021 BK005020 BK005019 BK005018 BK005017 BK005016 BK005015 BK005014 BK005013 BK005012 BK005011 BK005010 BK005009 BK005008 BK005007 BK005006 BK005027 BK005004 BK005005 BK005028 BK005029 BK005030 BK005031 BK005032 BK005033 BK005034 BK005035 BK005036 BK005037 BK005038 BK005039 BK005040
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase