Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Kanno,Y., Otomo,K., Kenmoku,H., Mitsuhashi,W., Yamane,H., Oikawa,H., Toshima,H., Matsuoka,M., Sassa,T. and Toyomasu,T. | |
| Title | Characterization of a rice gene family encoding type-a diterpene cyclases. | |
|
Abstract: We have previously isolated and characterized the rice (Oryza sativa) cDNAs, OsCyc1/OsCPS4, OsCyc2/OsCPS2, OsKS4, OsDTC1/OsKS7, OsDTC2/OsKS8 and OsKS10, which encode cyclases that are responsible for diterpene phytoalexin biosynthesis. Among the other members of this gene family, OsCPS1 and OsKS1 have been suggested as being responsible for gibberellin biosynthesis, OsKSL11 has recently been shown to encode stemodene synthase, and the functions of the three other diterpene cyclase genes in the rice genome, OsKS3, OsKS5 and OsKS6, have not yet been determined. In this study, we show that recombinant OsKS5 and OsKS6 expressed in E. coli converted ent-copalyl diphosphate into ent-pimara-8(14),15-diene and ent-kaur-15-ene, respectively. Neither product is a hydrocarbon precursor required in the biosynthesis of either gibberellins or phytoalexins. OsKS3 may be a pseudogene from which the translated product is a truncated enzyme. These results suggest that the diterpene cyclase genes responsible for gibberellin and phytoalexin biosynthesis are not functionally redundant. |
||
| Journal | Biosci. Biotechnol. Biochem. | |
| Country | Japan | |
| Volume | 70(7) | |
| Pages | 1702-1710 | |
| Year | 2006 | |
| PubMed ID | 16861806 | |
| PubMed Central ID | - | |
| DOI | 10.1271/bbb.60044 | |
| URL | - | |
| Relation | ||
| Gene | CPS1 CPS2 CPS4 KS1 KS3 KS4 KS5 KS6 KS7 KS8 KSL10 KSL11 | |
| INSD | AB243669 AB126936 AB126935 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
