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Author Cho,J.I., Ryoo,N., Ko,S., Lee,S.K., Lee,J., Jung,K.H., Lee,Y.H., Bhoo,S.H., Winderickx,J., An,G., Hahn,T.R. and Jeon,J.S.
Title Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.).
Abstract:
Hexokinase (HXK) is a dual-function enzyme that both phosphorylates hexose to form hexose 6-phosphate and plays an important role in sugar sensing and signaling. To investigate the roles of hexokinases in rice growth and development, we analyzed rice sequence databases and isolated ten rice hexokinase cDNAs, OsHXK1 (Oryza sativa Hexokinase 1) through OsHXK10. With the exception of the single-exon gene OsHXK1, the OsHXKs all have a highly conserved genomic structure consisting of nine exons and eight introns. Gene expression profiling revealed that OsHXK2 through OsHXK9 are expressed ubiquitously in various organs, whereas OsHXK10 expression is pollen-specific. Sugars induced the expression of three OsHXKs, OsHXK2, OsHXK5, and OsHXK6, in excised leaves, while suppressing OsHXK7 expression in excised leaves and immature seeds. The hexokinase activity of the OsHXKs was confirmed by functional complementation of the hexokinase-deficient yeast strain YSH7.4-3C (hxk1, hxk2, glk1). OsHXK4 was able to complement this mutant only after the chloroplast-transit peptide was removed. The subcellular localization of OsHXK4 and OsHXK7, observed with green fluorescent protein (GFP) fusion constructs, indicated that OsHXK4 is a plastid-stroma-targeted hexokinase while OsHXK7 localizes to the cytosol.
Journal Planta.
Country
Volume 224(3)
Pages 598-611
Year 2006
PubMed ID 16552590
PubMed Central ID -
DOI 10.1007/s00425-006-0251-y
URL -
Relation
Gene HXK1 HXK10 HXK2 HXK3 HXK4 HXK5 HXK6 HXK7 HXK8 HXK9
INSD DQ116383 DQ116384 DQ116385 DQ116386 DQ116387 DQ116388 DQ116389 DQ116390 DQ116391 DQ116392
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase