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Author Yuan,Q., Hill,J., Hsiao,J., Moffat,K., Ouyang,S., Cheng,Z., Jiang,J. and Buell,C.R.
Title Genome sequencing of a 239-kb region of rice chromosome 10L reveals a high frequency of gene duplication and a large chloroplast DNA insertion.
In this study we describe a 239-kb region on the long arm of rice chromosome 10 that contains a high density (71%) of locally duplicated genes, including 24 copies of a glutathione S-transferase gene. Intriguingly, embedded within this cluster is a large insertion (approximately 33 kb) of rice (Oryza sativa) chloroplast DNA that is derived from two separate regions of the chloroplast genome. We used DNA fiber-based fluorescence in situ hybridization (fiber-FISH) analyses of O. sativa spp. japonica nuclei to confirm that the insertion of organellar DNA was not a cloning artifact. The sequence of the chloroplast insertion is nearly identical (99.7% identity) to the corresponding regions in the published rice chloroplast genome sequence, suggesting that the transfer event occurred recently. PCR amplification and sequence analysis in two subspecies of rice, O. sativa spp. japonica and spp. indica, indicates that the transfer event predated the divergence of these two subspecies. The chloroplast insertion is flanked by a 2.1-kb perfect direct repeat that is unique to this location in the rice genome.
Journal Molecular Genetics & Genomics (Molecular General Genetics up to 2001)
Country USA
Volume 267(6)
Pages 713-720
Year 2002
PubMed ID 12207219
PubMed Central ID -
DOI 10.1007/s00438-002-0706-1
Gene GSTU12 GSTU13 GSTU14 GSTU15 GSTU18 GSTU19 GSTU2 GSTU20 GSTU22 GSTU23 GSTU24 GSTU25 GSTU26 GSTU27 GSTU28 GSTU3 GSTU30 GSTU4 GSTU8 GSTU9 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
INSD AC091680 AC074232
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -