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Reference Detail
| Reference | ||
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| Author | Ye,R., Yao,Q.H., Xu,Z.H. and Xue,H.W. | |
| Title | Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development. | |
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Abstract: Summary An efficient yeast-based system was developed for the isolation of plant cDNAs encoding transcription factors (TFs) and proteins with transcription activation functions (co-activators). The system consists of two vectors: (i) a reporter vector (pG221) harboring the iso-1-cytochrome c (CYC1) core promoter and the beta-galactosidase (lacZ) gene; and (ii) a cDNA library construction vector (pYF503), which yields a library of plant peptides fused to the GAL4-binding domain (GAL4-BD). Expression of a peptide harboring the characteristics of a transcriptional activator leads to expression of lacZ, allowing for selection of relevant colonies. TFs during rice embryo development were isolated through this system. Approximately 200 confirmed positive colonies were obtained from screening 10(6) yeast colonies, and sequence analysis of conserved domains identified 75 independent cDNAs, 20 of which encoded plant TFs or co-activators, including members of the APETALA2 (AP2)/ethylene-responsive element-binding protein (EREBP), MYB and growth-regulating factor (GRF) families. Peptides encoded by 13 of the isolated cDNAs were classified as potential TFs or co-activators because of the presence of conserved TF-like domains. Additionally, 2, 11, and 13 clones encoded kinases, chromosome-related proteins, and unknown proteins, respectively, while the remaining 16 cDNAs were associated with specific functions seemingly unrelated to TFs. Expression pattern analysis of selected TF-encoding genes via RT-PCR revealed that these genes were expressed during seed development, with differential transcription observed during various stages. This work provides informative hints for further study of the regulatory mechanism of rice seed development and illustrates an identification strategy that will be of practical value for the isolation of TFs and co-activators associated with specific plant developmental processes. |
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| Journal | Plant J. | |
| Country | China | |
| Volume | 38(2) | |
| Pages | 348-357 | |
| Year | 2004 | |
| PubMed ID | 15078336 | |
| PubMed Central ID | - | |
| DOI | 10.1111/j.1365-313X.2004.02037.x | |
| URL | - | |
| Relation | ||
| Gene | 2R_MYB23 C3H50 CBSCBS4 EIL1 ERF61 ERF68 ERF72 GRF5 GRF6 GS2 HOX5 HSFA9 MYB19 NAC91 NBR1 WRI1-1 WRKY37 XB15 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ | |
| INSD | AJ575240 AJ575241 AJ575242 AJ575243 AJ575244 AJ575245 AJ575223 AJ575222 AJ575221 AJ575220 AJ575217 AJ566408 AJ575219 AJ575218 AJ566409 AJ575224 AJ575225 AJ575226 AJ575227 AJ575228 AJ575229 AJ575230 AJ575231 AJ575232 AJ575233 AJ575234 AJ575235 AJ575236 AJ575237 AJ575238 AJ575239 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
