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著者		Yamanouchi,U., Yano,M., Lin,H., Ashikari,M. and Yamada,K.
タイトル		A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein.
Abstract: A rice spotted leaf (lesion-mimic) gene, Spl7, was identified by map-based cloning. High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for Spl7. We found one ORF that showed high similarity to a heat stress transcription factor (HSF). Transgenic analysis verified the function of the candidate gene for Spl7: leaf spot development was suppressed in spl7 mutants with a wild-type Spl7 transgene. Thus, we conclude that Spl7 encodes the HSF protein. The transcript of spl7 was observed in mutant plants. The levels of mRNAs (Spl7 in wild type and spl7 in mutant) increased under heat stress. Sequence analysis revealed only one base substitution in the HSF DNA-binding domain of the mutant allele, causing a change from tryptophan to cysteine.
掲載誌		Proc. Natl. Acad. Sci. U.S.A.
Country		Japan
刊数		99(11)
ページ		7530-7535
発刊年		2002
PubMed ID		12032317
PubMed Central ID		-
DOI		10.1073/pnas.112209199
URL		-
関連情報
遺伝子		SPL7
INSD		AB050095 AB050096 AB050097 AB050098
系統	野生イネコアコレクション	-
	突然変異系統(遺伝研 Collection)	-
	種子不稔系統	-
	致死変異体系統	-
	組織別発生段階 - 突然変異系統(遺伝子)	-
	栽培イネ品種(遺伝研 Collection)	-
組織別発生段階		-