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Reference Detail
| Reference | ||
|---|---|---|
| Author | Umeda,M., Hara,C., Matsubayashi,Y., Li,H.H., Liu,Q., Tadokoro,F., Aotsuka,S. and Uchimiya,H. | |
| Title | Expressed sequence tags from cultured cells of rice (Oryza sativa L.) under stressed conditions: analysis of transcripts of genes engaged in ATP-generating pathways. | |
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Abstract: Large-scale sequencing of randomly selected cDNA clones was used to isolate numerous genes in rice (Oryza sativa L.). Total RNA used for cDNA synthesis was prepared from suspension-cultured cells of rice grown under stressed conditions, such as in saline or nitrogen-starvation conditions. A total of 780 cDNA clones were partially sequenced and about 15% could be identified as putative genes. In the library constructed under saline conditions, we identified several genes associated with signal transduction, such as protein kinase and small GTP-binding protein genes. Many stress-related genes were isolated from both the saline and nitrogen-starvation libraries. These results indicate that stress treatment of suspension-cultured cells makes it possible to efficiently isolate various types of plant genes. To examine the usefulness of such tagged cDNAs for the study of gene expression in a specific metabolic pathway, we analyzed mRNA levels of genes engaged in the ATP-generating pathways in cultured cells of rice under different stresses, such as 20% sucrose, salt stress, cold stress and nitrogen-starvation stress. The results suggest that the coordinated induction of several genes in key steps under stressed conditions may be essential for activation of the entire energy-producing pathway to maintain homeostasis in rice cells. Expressed sequence tags identified by random cDNA sequencing provide the opportunity to generate a transcript map of rice genes. |
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| Journal | Plant Mol. Biol. | |
| Country | Japan | |
| Volume | 25(3) | |
| Pages | 469-478 | |
| Year | 1994 | |
| PubMed ID | 8049371 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | 25S rRNA ADH1 FBA2 GAPC1 LPS1 PDC1 PFPB PGI1 PGK4 PK3 TPI _ _ _ _ _ | |
| INSD | D21278 D10408 D10409 D10411 D10412 D10413 D10414 D10415 D10419 D10961 D17765 D17766 D17768 D17769 D17770 D21069 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
