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| 文献 | ||
|---|---|---|
| 著者 | Takahashi,Y., Shomura,A., Sasaki,T. and Yano,M. | |
| タイトル | Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2. | |
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Abstract: Hd6 is a quantitative trait locus involved in rice photoperiod sensitivity. It was detected in backcross progeny derived from a cross between the japonica variety Nipponbare and the indica variety Kasalath. To isolate a gene at Hd6, we used a large segregating population for the high-resolution and fine-scale mapping of Hd6 and constructed genomic clone contigs around the Hd6 region. Linkage analysis with P1-derived artificial chromosome clone-derived DNA markers delimited Hd6 to a 26.4-kb genomic region. We identified a gene encoding the alpha subunit of protein kinase CK2 (CK2 alpha) in this region. The Nipponbare allele of CK2 alpha contains a premature stop codon, and the resulting truncated product is undoubtedly nonfunctional. Genetic complementation analysis revealed that the Kasalath allele of CK2 alpha increases days-to-heading. Map-based cloning with advanced backcross progeny enabled us to identify a gene underlying a quantitative trait locus even though it exhibited a relatively small effect on the phenotype. |
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| 掲載誌 | Proc. Natl. Acad. Sci. U.S.A. | |
| Country | Japan | |
| 刊数 | 98(14) | |
| ページ | 7922-7927 | |
| 発刊年 | 2001 | |
| PubMed ID | 11416158 | |
| PubMed Central ID | - | |
| DOI | 10.1073/pnas.111136798 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | HD16 HD6 | |
| INSD | AB036785 AB036786 AB036787 AB036788 | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
