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Author Prasad,K., Kushalappa,K. and Vijayraghavan,U.
Title Mechanism underlying regulated expression of RFL, a conserved transcription factor, in the developing rice inflorescence.
Abstract:
LFY and its orthologues are necessary for flower specification in diverse dicotyledonous plants. The spatial and temporal RNA expression pattern of a rice LFY-like gene: RFL differs significantly from that in several other species studied thus far. The onset of RFL expression coincides with inflorescence meristem (panicle meristem) initiation, and continues during panicle branching. Notably, incipient flower primordia have lower expression levels, and during floral organogenesis the expression is restricted to second-whorl lodicules. To explore mechanisms underlying this distinct expression pattern, we have tested the transcription regulatory functions of sequences upstream to RFL coding sequences either alone, or together with intragenic segments. Sequences 3.0 kb upstream of the RFL reading frame do not confer correctly regulated reporter gene expression in transgenic rice. In contrast, RFL intron1 or 2 can individually confer the expected profile in the developing panicle and floret. However, the additional repression of expression in vegetative tissues, is a pattern achieved by intron2 together with far-upstream sequences. Strikingly, RFL intron2 sequences can even utilize the Arabidopsis thaliana LFY promoter to confer regulated transcription in young rice panicles. By sequence comparison of RFL upstream sequences, intron1, intron2 and the Arabidopsis LFY promoter, we identify putative cis-regulatory elements unique to RFL. These data exemplify the use of regulatory circuits specific to rice RFL that may underlie its divergent function.
Journal Mech. Dev.
Country India
Volume 120(4)
Pages 491-502
Year 2003
PubMed ID 12676326
PubMed Central ID -
DOI 10.1016/S0925-4773(02)00457-4
URL -
Relation
Gene RFL
INSD AF397034
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase