Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Nemoto,T., Cho,E.M., Okada,A., Okada,K., Otomo,K., Kanno,Y., Toyomasu,T., Mitsuhashi,W., Sassa,T., Minami,E., Shibuya,N., Nishiyama,M., Nojiri,H. and Yamane,H. | |
| Title | Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice. | |
|
Abstract: In suspension-cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension-cultured rice cells treated with a chitin elicitor. The OsDTC2 cDNA was overexpressed in Escherichia coli as a fusion protein with glutathione S-transferase, and the recombinant OsDTC2 was indicated to function as stemar-13-ene synthase that converted syn-copalyl diphosphate to stemar-13-ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of OsDTC2 mRNA in suspension-cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of OsDTC2 was also induced in UV-irradiated rice leaves. In addition, we indicated that stemar-13-ene accumulated in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. |
||
| Journal | FEBS Lett. | |
| Country | Japan | |
| Volume | 571(1-3) | |
| Pages | 182-186 | |
| Year | 2004 | |
| PubMed ID | 15280039 | |
| PubMed Central ID | - | |
| DOI | 10.1016/j.febslet.2004.07.002 | |
| URL | - | |
| Relation | ||
| Gene | CPS2 CPS4 KS8 | |
| INSD | AB118056 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
