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Reference Detail
| Reference | ||
|---|---|---|
| Author | Nantel,A. and Quatrano,R.S. | |
| Title | Characterization of three rice basic/leucine zipper factors, including two inhibitors of EmBP-1 DNA binding activity. | |
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Abstract: The promoter of the wheat Em gene contains elements with a CACGTG core sequence (G-boxes), which are recognized by EmBP-1, a wheat basic/leucine zipper (bZIP) protein. G-boxes are required for Em expression in response to the phytohormone abscisic acid and for transactivation by the Viviparous-1 protein (VP1) using transient expression systems. In order to identify other factors that are part of the transcriptional complex that associates with G-boxes, we have screened a rice (Oryza sativa) cDNA library with biotinylated EmBP-1. We have isolated osZIP-1a, a homolog of EmBP-1 and other plant G-box-binding factors. We show that EmBP-1 and osZIP-1a will preferentially heterodimerize in vitro. Overexpression of osZIP-1a in rice protoplasts can enhance expression from the Em promoter only in the presence of abscisic acid. Two other clones have been identified by screening with EmBP-1: osZIP-2a and osZIP-2b. These osZIP-2 factors represent a novel class of bZIP proteins with an unusual DNA-binding domain that does not recognize G-boxes. The osZIP-2 factors can heterodimerize with EmBP-1 and prevent it from binding to the Em promoter. Interestingly, osZIP-1a does not heterodimerize with the osZIP-2 factors and its DNA binding activity is unaffected by their presence. Thus, osZIP-2 factors may be involved in sequestering a particular group of G-box-binding factors into inactive heterodimers. |
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| Journal | J. Biol. Chem. | |
| Country | USA | |
| Volume | 271(49) | |
| Pages | 31296-31305 | |
| Year | 1996 | |
| PubMed ID | 8940135 | |
| PubMed Central ID | - | |
| DOI | 10.1074/jbc.271.49.31296 | |
| URL | - | |
| Relation | ||
| Gene | ZIP1 ZIP1A ZIP2 ZIP2A _ | |
| INSD | U04295 U04296 U04297 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
