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Reference Detail
| Reference | ||
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| Author | Moriguchi,K., Suzuki,T., Ito,Y., Yamazaki,Y., Niwa,Y. and Kurata,N. | |
| Title | Functional isolation of novel nuclear proteins showing a variety of subnuclear localizations. | |
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Abstract: Nuclear proteins play key roles in the fundamental regulation of genome instability, the phases of organ development, and physiological responsiveness through gene expression. Although nuclear proteins have been shown to account for approximately one-fourth of total proteins in yeast, no efficient method to identify novel nuclear proteins has been applied to plants. In this study, a trial to isolate nuclear proteins in rice was attempted, and several novel nuclear proteins showing a variety of subnuclear localizations were identified. The nuclear transportation trap (NTT) system, which is a modified two-hybrid system, isolated many nuclear proteins from rice (Oryza sativa) NTT cDNA libraries. Nuclear localization of the isolated proteins was confirmed by transient introduction of green fluorescent protein fusion constructs for a subset of protein genes into onion (Allium cepa) cells. The majority of these proteins, including novel proteins and proteins initially categorized as cytoplasmic proteins, were revealed to be localized in the nucleus. Detailed characterization of unknown proteins revealed various subnuclear localizations, indicating their possible association with chromatin and the nuclear matrix with a foci or speckle-like distribution. Some also showed dual distribution in the nucleus and cytoplasm. In the novel protein fraction, a protein was further identified for its chromatin-associated localization in a specific organ of rice by immunostaining. Thus, a variety of novel nuclear architectural proteins with chromatin or matrix associating abilities, which are important in nuclear organization by influencing certain organ developments or cell responsiveness, can be isolated using the NTT method. Because nuclear proteins other than transcription regulators have rarely been characterized in plants, such as matrix proteins and development-specific chromatin proteins, their identification and subsequent characterization could provide important information for genome-wide regulatory mechanisms controlled by nuclear organization. |
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| Journal | Plant Cell | |
| Country | Japan | |
| Volume | 17(2) | |
| Pages | 389-403 | |
| Year | 2005 | |
| PubMed ID | 15659629 | |
| PubMed Central ID | - | |
| DOI | 10.1105/tpc.104.028456 | |
| URL | - | |
| Relation | ||
| Gene | AHP1 NCED1 SCL30B _ _ _ _ _ | |
| INSD | AB110172 AB110173 AB110174 AB110175 AB110176 AB110177 AB110178 AB110179 AB110180 AB110181 AB110182 AB110183 AB110184 AB110185 AB110186 AB110187 AB110188 AB110189 AB110190 AB110191 AB110192 AB110193 AB110194 AB110195 AB110196 AB110197 AB110198 AB110199 AB110200 AB110201 AB110202 AB110203 AB110204 AB110205 AB110206 AB110207 AB110164 AB110165 AB110166 AB110167 AB110168 AB110169 AB110170 AB110171 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
