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| 著者 | Lee,S., Kim,J., Son,J.S., Nam,J., Jeong,D.H., Lee,K., Jang,S., Yoo,J., Lee,J., Lee,D.Y., Kang,H.G. and An,G. | |
| タイトル | Systematic Reverse Genetic Screening of T-DNA Tagged Genes in Rice for Functional Genomic Analyses: MADS-box Genes as a Test Case. | |
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Abstract: We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to genean@postech.ac.kr. |
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| 掲載誌 | Plant Cell Physiol. | |
| Country | South Korea | |
| 刊数 | 44(12) | |
| ページ | 1403-1411 | |
| 発刊年 | 2003 | |
| PubMed ID | 14701936 | |
| PubMed Central ID | - | |
| DOI | 10.1093/pcp/pcg156 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | CFO1 COG1 FST LHS1 MADS13 MADS18 MADS2 MADS20 MADS21 MADS22 MADS23 MADS24 MADS25 MADS26 MADS27 MADS30 MADS31 MADS33 MADS35 MADS36 MADS4 MADS47 MADS5 MADS50 MADS55 MADS56 MADS57 MADS7 MFO1 PAP2 RAP1A RAP1B | |
| INSD | AY177696 AY177697 AY177699 AY177700 AY177702 AY250075 AY345220 AY345221 AY345222 AY345223 AY345224 AY174093 AY177693 AY177695 | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
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| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
