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Reference
Author Lee,S., Kim,J., Son,J.S., Nam,J., Jeong,D.H., Lee,K., Jang,S., Yoo,J., Lee,J., Lee,D.Y., Kang,H.G. and An,G.
Title Systematic Reverse Genetic Screening of T-DNA Tagged Genes in Rice for Functional Genomic Analyses: MADS-box Genes as a Test Case.
Abstract:
We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to genean@postech.ac.kr.
Journal Plant Cell Physiol.
Country South Korea
Volume 44(12)
Pages 1403-1411
Year 2003
PubMed ID 14701936
PubMed Central ID -
DOI 10.1093/pcp/pcg156
URL -
Relation
Gene FST LHS1 MADS13 MADS17 MADS18 MADS2 MADS20 MADS21 MADS22 MADS23 MADS24 MADS25 MADS26 MADS27 MADS30 MADS31 MADS32 MADS33 MADS35 MADS36 MADS4 MADS47 MADS5 MADS50 MADS55 MADS56 MADS57 MADS7 MFO1 PAP2 RAP1A RAP1B
INSD AY177696 AY177697 AY177699 AY177700 AY177702 AY250075 AY345220 AY345221 AY345222 AY345223 AY345224 AY174093 AY177693 AY177695
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase