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| 文献 | ||
|---|---|---|
| 著者 | Komori,T., Ohta,S., Murai,N., Takakura,Y., Kuraya,Y., Suzuki,S., Hiei,Y., Imaseki,H. and Nitta,N. | |
| タイトル | Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.). | |
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Abstract: A rice nuclear gene, Rf-1, restores the pollen fertility disturbed by the BT-type male sterile cytoplasm, and is widely used for commercial seed production of japonica hybrid varieties. Genomic fragments carrying Rf-1 were identified by conducting chromosome walking and a series of complementation tests. Isolation and analysis of cDNA clones corresponding to the fragments demonstrated that Rf-1 encodes a mitochondrially targeted protein containing 16 repeats of the 35-aa pentatricopeptide repeat (PPR) motif. Sequence analysis revealed that the recessive allele, rf-1, lacks one nucleotide in the putative coding region, presumably resulting in encoding a truncated protein because of a frame shift. Rice Rf-1 is the first restorer gene isolated from cereal crops that has the property of reducing the expression of the cytoplasmic male sterility (CMS)-associated mitochondrial gene like many other restorer genes. The present findings may facilitate not only elucidating the mechanisms of male sterility by the BT cytoplasm and its restoration by Rf-1 but also isolating other restorer genes from cereal crops, especially rice. |
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| 掲載誌 | Plant J. | |
| Country | Japan | |
| 刊数 | 37(3) | |
| ページ | 315-325 | |
| 発刊年 | 2004 | |
| PubMed ID | 14731253 | |
| PubMed Central ID | - | |
| DOI | 10.1046/j.1365-313X.2003.01961.x | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | RF1 _ _ _ | |
| INSD | AB110444 AB110443 | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
rf1 | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
