研究支援ツール - 文献 - 詳細
文献詳細
文献 | ||
---|---|---|
著者 | Komori,T., Ohta,S., Murai,N., Takakura,Y., Kuraya,Y., Suzuki,S., Hiei,Y., Imaseki,H. and Nitta,N. | |
タイトル | Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.). | |
Abstract: A rice nuclear gene, Rf-1, restores the pollen fertility disturbed by the BT-type male sterile cytoplasm, and is widely used for commercial seed production of japonica hybrid varieties. Genomic fragments carrying Rf-1 were identified by conducting chromosome walking and a series of complementation tests. Isolation and analysis of cDNA clones corresponding to the fragments demonstrated that Rf-1 encodes a mitochondrially targeted protein containing 16 repeats of the 35-aa pentatricopeptide repeat (PPR) motif. Sequence analysis revealed that the recessive allele, rf-1, lacks one nucleotide in the putative coding region, presumably resulting in encoding a truncated protein because of a frame shift. Rice Rf-1 is the first restorer gene isolated from cereal crops that has the property of reducing the expression of the cytoplasmic male sterility (CMS)-associated mitochondrial gene like many other restorer genes. The present findings may facilitate not only elucidating the mechanisms of male sterility by the BT cytoplasm and its restoration by Rf-1 but also isolating other restorer genes from cereal crops, especially rice. |
||
掲載誌 | Plant J. | |
Country | Japan | |
刊数 | 37(3) | |
ページ | 315-325 | |
発刊年 | 2004 | |
PubMed ID | 14731253 | |
PubMed Central ID | - | |
DOI | 10.1046/j.1365-313X.2003.01961.x | |
URL | - | |
関連情報 | ||
遺伝子 | RF1 _ _ _ | |
INSD | AB110444 AB110443 | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
rf1 | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |