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Reference Detail
| Reference | ||
|---|---|---|
| Author | Komori,T., Ohta,S., Murai,N., Takakura,Y., Kuraya,Y., Suzuki,S., Hiei,Y., Imaseki,H. and Nitta,N. | |
| Title | Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.). | |
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Abstract: A rice nuclear gene, Rf-1, restores the pollen fertility disturbed by the BT-type male sterile cytoplasm, and is widely used for commercial seed production of japonica hybrid varieties. Genomic fragments carrying Rf-1 were identified by conducting chromosome walking and a series of complementation tests. Isolation and analysis of cDNA clones corresponding to the fragments demonstrated that Rf-1 encodes a mitochondrially targeted protein containing 16 repeats of the 35-aa pentatricopeptide repeat (PPR) motif. Sequence analysis revealed that the recessive allele, rf-1, lacks one nucleotide in the putative coding region, presumably resulting in encoding a truncated protein because of a frame shift. Rice Rf-1 is the first restorer gene isolated from cereal crops that has the property of reducing the expression of the cytoplasmic male sterility (CMS)-associated mitochondrial gene like many other restorer genes. The present findings may facilitate not only elucidating the mechanisms of male sterility by the BT cytoplasm and its restoration by Rf-1 but also isolating other restorer genes from cereal crops, especially rice. |
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| Journal | Plant J. | |
| Country | Japan | |
| Volume | 37(3) | |
| Pages | 315-325 | |
| Year | 2004 | |
| PubMed ID | 14731253 | |
| PubMed Central ID | - | |
| DOI | 10.1046/j.1365-313X.2003.01961.x | |
| URL | - | |
| Relation | ||
| Gene | RF1 _ _ _ | |
| INSD | AB110444 AB110443 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
rf1 | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
