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Author Komori,T., Ohta,S., Murai,N., Takakura,Y., Kuraya,Y., Suzuki,S., Hiei,Y., Imaseki,H. and Nitta,N.
Title Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.).
A rice nuclear gene, Rf-1, restores the pollen fertility disturbed by the BT-type male sterile cytoplasm, and is widely used for commercial seed production of japonica hybrid varieties. Genomic fragments carrying Rf-1 were identified by conducting chromosome walking and a series of complementation tests. Isolation and analysis of cDNA clones corresponding to the fragments demonstrated that Rf-1 encodes a mitochondrially targeted protein containing 16 repeats of the 35-aa pentatricopeptide repeat (PPR) motif. Sequence analysis revealed that the recessive allele, rf-1, lacks one nucleotide in the putative coding region, presumably resulting in encoding a truncated protein because of a frame shift. Rice Rf-1 is the first restorer gene isolated from cereal crops that has the property of reducing the expression of the cytoplasmic male sterility (CMS)-associated mitochondrial gene like many other restorer genes. The present findings may facilitate not only elucidating the mechanisms of male sterility by the BT cytoplasm and its restoration by Rf-1 but also isolating other restorer genes from cereal crops, especially rice.
Journal Plant J.
Country Japan
Volume 37(3)
Pages 315-325
Year 2004
PubMed ID 14731253
PubMed Central ID -
DOI 10.1046/j.1365-313X.2003.01961.x
Gene RF1 _ _ _
INSD AB110444 AB110443
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -