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Reference Detail
| Reference | ||
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| Author | Iwabuchi,M., Kyozuka,J. and Shimamoto,K. | |
| Title | Processing followed by complete editing of an altered mitochondrial atp6 RNA restores fertility of cytoplasmic male sterile rice. | |
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Abstract: Two atp6 genes were found in the mitochondrial genome of cytoplasmic male sterile (CMS) rice carrying the [cms-bo] cytoplasm. One (N-atp6) was identical to the normal cytoplasmic gene, while the second (B-atp6) was identified as a candidate CMS gene by Southern analysis of the mitochondrial genome of CMS cybrid rice. The coding sequence of B-atp6 was identical to the normal N-atp6 gene but its 3'-flanking sequence was different starting at 49 bases downstream from the stop codon. Northern analysis showed that B-atp6 is transcribed into a 2.0 kb RNA in the absence of the Rf-1 gene, whereas two discontinuous RNAs, of approximately 1.5 and 0.45 kb, were detected in the presence of the Rf-1 gene. Determination of the 3' and 5' ends of these RNAs suggested that the two discontinuous RNAs were generated from the 2.0 kb RNA by RNA processing at sites within the B-atp6-specific sequences by the action of the Rf-1 gene. Sequence analysis of cDNA clones derived from the N-atp6 RNA and the processed and unprocessed RNAs of B-atp6 indicated that the processed B-atp6 RNAs were edited as efficiently as the N-atp6, whereas unedited and partially edited RNAs were detected among unprocessed RNAs. RNA processing by Rf-1 thus influences the sequential post-transcriptional editing of the B-atp6 RNAs. Because the unprocessed RNAs of B-atp6 are possibly translated into altered polypeptides, our results suggest that interaction of RNA processing and editing plays a role in controlling CMS expression and the restoration of fertility in rice. |
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| Journal | EMBO J. | |
| Country | Japan | |
| Volume | 12(4) | |
| Pages | 1437-1446 | |
| Year | 1993 | |
| PubMed ID | 7682174 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | AATP6 _ | |
| INSD | S59393 S59890 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
