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| Reference | ||
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| Author | Ishimaru,Y., Suzuki,M., Tsukamoto,T., Suzuki,K., Nakazono,M., Kobayashi,T., Wada,Y., Watanabe,S., Matsuhashi,S., Takahashi,M., Nakanishi,H., Mori,S. and Nishizawa,N.K. | |
| Title | Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+ | |
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Abstract: Only graminaceous monocots possess the Strategy II iron (Fe)-uptake system in which Fe is absorbed by roots as an Fe3+-phytosiderophore. In spite of being a Strategy II plant, however, rice (Oryza sativa) contains the previously identified Fe2+ transporter OsIRT1. In this study, we isolated the OsIRT2 gene from rice, which is highly homologous to OsIRT1. Real-time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low-Fe conditions. When expressed in yeast (Saccharomyces cerevisiae) cells, OsIRT2 cDNA reversed the growth defects of a yeast Fe-uptake mutant. This was similar to the effect of OsIRT1 cDNA. OsIRT1- and OsIRT2-green fluorescent protein fusion proteins localized to the plasma membrane when transiently expressed in onion (Allium cepa L.) epidermal cells. OsIRT1 promoter-GUS analysis revealed that OsIRT1 is expressed in the epidermis and exodermis of the elongating zone and in the inner layer of the cortex of the mature zone of Fe-deficient roots. OsIRT1 expression was also detected in the ccompanion cells. Analysis using the positron-emitting tracer imaging system showed that rice plants are able to take up both an Fe3+-phytosiderophore and Fe2+. This result indicates that, in addition to absorbing an Fe3+-phytosiderophore, rice possesses a novel Fe-uptake system that directly absorbs the Fe2+, a strategy that is advantageous for growth in submerged conditions. |
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| Journal | Plant J. | |
| Country | Japan | |
| Volume | 45(3) | |
| Pages | 335-346 | |
| Year | 2006 | |
| PubMed ID | 16412081 | |
| PubMed Central ID | - | |
| DOI | 10.1111/j.1365-313X.2005.02624.x | |
| URL | - | |
| Relation | ||
| Gene | FRO2 IRT1 IRT2 ZIP1 ZIP2 ZIP3 ZIP4 ZIP5 ZIP6 ZIP7 _ | |
| INSD | AB126085 AB126086 AB126084 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
