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Author Igarashi,Y., Yoshiba,Y., Takeshita,T., Nomura,S., Otomo,J., Yamaguchi-Shinozaki,K. and Shinozaki,K.
Title Molecular cloning and characterization of a cDNA encoding proline transporter in rice.
Abstract:
A cDNA encoding a proline (Pro) transporter (ProT) was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the rice ProT protein (OsProT) had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculentum. Northern blot analysis revealed that the gene for OsProT (OsProT) was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for delta1-pyrroline-5-carboxylate synthetase (P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported L-Pro in a transport assay using Xenopus laevis oocytes.
Journal Plant Cell Physiol.
Country Japan
Volume 41(6)
Pages 750-756
Year 2000
PubMed ID 10945345
PubMed Central ID -
DOI -
URL -
Relation
Gene P5CS _
INSD AB022783
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase