研究支援ツール - 文献 - 詳細

文献詳細

文献
著者 Igarashi,Y., Yoshiba,Y., Takeshita,T., Nomura,S., Otomo,J., Yamaguchi-Shinozaki,K. and Shinozaki,K.
タイトル Molecular cloning and characterization of a cDNA encoding proline transporter in rice.
Abstract:
A cDNA encoding a proline (Pro) transporter (ProT) was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the rice ProT protein (OsProT) had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculentum. Northern blot analysis revealed that the gene for OsProT (OsProT) was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for delta1-pyrroline-5-carboxylate synthetase (P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported L-Pro in a transport assay using Xenopus laevis oocytes.
掲載誌 Plant Cell Physiol.
Country Japan
刊数 41(6)
ページ 750-756
発刊年 2000
PubMed ID 10945345
PubMed Central ID -
DOI -
URL -
関連情報
遺伝子 P5CS PROT
INSD AB022783
系統 野生イネ
コアコレクション
-
突然変異系統(遺伝研 Collection) -
種子不稔系統 -
致死変異体系統 -
組織別発生段階
- 突然変異系統(遺伝子)
-
栽培イネ品種(遺伝研 Collection) -
組織別発生段階 -
/rice/oryzabase