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Reference Detail
| Reference | ||
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| Author | Cho,E., Okada,A., Kenmoku,H., Otomo,K., Toyomasu,T., Mitsuhashi,W., Sassa,T., Yajima,A., Yabuta,G., Mori,K., Oikawa,H., Toshima,H., Shibuya,N., Nojiri,H., Omori,T., Nishiyama,M. and Yamane,H. | |
| Title | Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor. | |
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Abstract: We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice. |
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| Journal | Plant J. | |
| Country | Japan | |
| Volume | 37(1) | |
| Pages | 1-8 | |
| Year | 2004 | |
| PubMed ID | 14675427 | |
| PubMed Central ID | - | |
| DOI | 10.1046/j.1365-313X.2003.01926.x | |
| URL | - | |
| Relation | ||
| Gene | KS7 KS8 | |
| INSD | AB089272 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
