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Reference Detail
| Reference | ||
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| Author | Banuelos,M.A., Garciadeblas,B., Cubero,B. and Rodriguez-Navarro,A. | |
| Title | Inventory and functional characterization of the HAK potassium transporters of rice. | |
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Abstract: Plants take up large amounts of K(+) from the soil solution and distribute it to the cells of all organs, where it fulfills important physiological functions. Transport of K(+) from the soil solution to its final destination is mediated by channels and transporters. To better understand K(+) movements in plants, we intended to characterize the function of the large KT-HAK-KUP family of transporters in rice (Oryza sativa cv Nipponbare). By searching in databases and cDNA cloning, we have identified 17 genes (OsHAK1-17) encoding transporters of this family and obtained evidence of the existence of other two genes. Phylogenetic analysis of the encoded transporters reveals a great diversity among them, and three distant transporters, OsHAK1, OsHAK7, and OsHAK10, were expressed in yeast (Saccharomyces cerevisiae) and bacterial mutants to determine their functions. The three transporters mediate K(+) influxes or effluxes, depending on the conditions of the experiment. A comparative kinetic analysis of HAK-mediated K(+) influx in yeast and in roots of K(+)-starved rice seedlings demonstrated the involvement of HAK transporters in root K(+) uptake. We discuss that all HAK transporters may mediate K(+) transport, but probably not only in the plasma membrane. Transient expression of the OsHAK10-green fluorescent protein fusion protein in living onion epidermal cells targeted this protein to the tonoplast. |
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| Journal | Plant Physiol. | |
| Country | Spain | |
| Volume | 130(2) | |
| Pages | 784-795 | |
| Year | 2002 | |
| PubMed ID | 12376644 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.007781 | |
| URL | - | |
| Relation | ||
| Gene | HAK10 HAK11 HAK12 HAK13 HAK14 HAK15 HAK16 HAK17 HAK2 HAK3 HAK4 HAK5 HAK6 HAK7 HAK8 HAK9 LCS | |
| INSD | AJ427970 AJ427971 AJ427972 AJ427973 AJ427974 AJ427975 AJ427976 AJ427977 AJ427978 AJ427979 AJ427980 AJ427981 AJ427982 AJ427983 AJ427984 | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
