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著者 | Agrawal,G.K., Jwa,N.S., Shibato,J., Han,O., Iwahashi,H. and Rakwal,R. | |
タイトル | Diverse environmental cues transiently regulate OsOPR1 of the "octadecanoid pathway" revealing its importance in rice defense/stress and development. | |
Abstract: Previously, we reported rice (Oryza sativa L. japonica type cv. Nipponbare) allene oxide synthase (OsAOS) and cyclase (OsAOC) genes of the octadecanoid pathway. Here we have isolated a 12-oxo-phytodienoic acid reductase gene, called OsOPR1, encoding the last committed enzymatic step on the octadecanoid pathway leading to jasmonic acid (JA) biosynthesis. OsOPR1 encodes a 380 amino acid long polypeptide with a predicted molecular mass of 42465.02 and pI of 5.79, and belongs to a gene family in the rice genome. Transcriptional profiling using our established two-week-old rice seedling model system against a variety of environmental factors such as wounding, global signals (including JA), osmotic stresses, heavy metals, UV-C irradiation, fungal elicitor, protein phosphatase inhibitors, and gaseous pollutants indicated that OsOPR1 transcript was rapidly, transiently and differentially up-regulated within 30 min in leaves. Surprisingly, co-application of signaling molecules JA, salicylate and ethylene, resulted in a massive accumulation of the OsOPR1 transcript at 30 min and remained elevated with time, a new observation. Furthermore, transient expression of OsOPR1, most likely regulated by a de novo synthesized negative trans-acting factor(s), was evidenced by the use of cycloheximide. Finally, the endogenous OsOPR1 expression varied with the stage of plant development. These results strongly suggest a regulatory role for OsOPR1 in rice plant defense/stress response pathway(s) and reproduction. |
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掲載誌 | Biochem. Biophys. Res. Commun. | |
Country | ||
刊数 | 310(4) | |
ページ | 1073-1082 | |
発刊年 | 2003 | |
PubMed ID | 14559225 | |
PubMed Central ID | - | |
DOI | - | |
URL | - | |
関連情報 | ||
遺伝子 | AOC AOS1 OG1 OPR1 OPR10 OPR4 OPR5 OPR6 OPR8 | |
INSD | AJ557138 AJ557139 | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |