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Author Nonomura, K.I., Nakano, M., Murata, K., Miyoshi, K., Eiguchi, M. et al.
Title An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis.
Abstract:
To elucidate the genetic system that establishes homologous chromosome pairing in monocot plants, we have isolated an asynaptic mutant of rice, designated pair2 (homologous pairing aberration in rice meiosis 2), in which 24 completely unpaired univalents are observed at pachytene and diakinesis. The mutation was caused by an insertion of the retrotransposon Tos17, as demonstrated by complementation of the mutation by transformation with the corresponding wild-type gene. The gene in which the element was inserted is orthologous to the ASY1 gene of Arabidopsis thaliana and the HOP1 gene of Saccharomyces cerevisiae. Mature PAIR2 mRNA and several splicing variants were found to be highly expressed in wild-type reproductive tissues, and lower expression was also detected in vegetative tissues. In situ hybridization and BrdU incorporation experiments revealed that PAIR2 expression is specifically enhanced in male and female meiocytes, but not in those at pre-meiotic S phase or in the pollen maturation stages. The results obtained in this study suggest that the PAIR2 gene is essential for homologous chromosome pairing in meiosis, as in the case of the genes ASY1 and HOP1. The study also suggested the possibility that a highly homologous copy of the PAIR2 gene located on a different chromosome is in fact a pseudogene.
Journal Molecular Genetics & Genomics (Molecular General Genetics up to 2001)
Country
Volume 271
Pages 121-129
Year 2004
PubMed ID 14758540
PubMed Central ID -
DOI 10.1007/s00438-003-0934-z
URL -
Relation
Gene PAIR2
INSD -
Strain Wild Core Collection -
Sterile Seed Strain STER-NIG0007
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
pair2
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase