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Reference Detail
| Reference | ||
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| Author | Sakamoto, T., K. Miura, H. Itoh, T. Tatsumi, M. Ueguchi-Tanaka, K. Ishiyama, M. Kobayashi, G.K. Agrawal, S. Takeda, K. Abe, A. Miyao, H. Hirochika, H. Kitano, M. Ashikari and M. Matsuoka | |
| Title | An overview of gibberellin metabolism enzyme genes and their related mutants in rice. | |
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Abstract: To enhance our understanding of GA metabolism in rice (Oryza sativa), we intensively screened and identified 29 candidate genes encoding the following GA metabolic enzymes using all available rice DNA databases: ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurene oxidase (KO), ent-kaurenoic acid oxidase (KAO), GA 20-oxidase (GA20ox), GA 3-oxidase (GA3ox), and GA 2-oxidase (GA2ox). In contrast to the Arabidopsis genome, multiple CPS-like, KS-like, and KO-like genes were identified in the rice genome, most of which are contiguously arranged. We also identified 18 GA-deficient rice mutants at six different loci from rice mutant collections. Based on the mutant and expression analyses, we demonstrated that the enzymes catalyzing the early steps in the GA biosynthetic pathway (i.e. CPS, KS, KO, and KAO) are mainly encoded by single genes, while those for later steps (i.e. GA20ox, GA3ox, and GA2ox) are encoded by gene families. The remaining CPS-like, KS-like, and KO-like genes were likely to be involved in the biosynthesis of diterpene phytoalexins rather than GAs because the expression of two CPS-like and three KS-like genes (OsCPS2, OsCPS4, OsKS4, OsKS7, and OsKS8) were increased by UV irradiation, and four of these genes (OsCPS2, OsCPS4, OsKS4, and OsKS7) were also induced by an elicitor treatment. |
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| Journal | Plant Physiol. | |
| Country | Japan | |
| Volume | 134 | |
| Pages | 1642-1653 | |
| Year | 2004 | |
| PubMed ID | 15075394 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.103.033696 | |
| URL | - | |
| Relation | ||
| Gene | CPS1 CPS2 CPS3 CPS4 D18 D35 GA20OX3 GA2OX1 GA2OX2 GA2OX3 GA3OX1 GNP1 KAO KO1 KO3 KO4 KO5 KS1 KS2 KS3 KS4 KS5 KS6 KS7 KS8 KS9 PRL5 SBI SD1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
