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| 文献 | ||
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| 著者 | Kamiya, N., H. Nagasaki, A. Morikami, Y. Sato and M. Matsuoka | |
| タイトル | Isolation and characterization of a rice WUSCHEL-type homeobox gene that is specefically expressed in the central cells of a quiescent center in the root apical meristem. | |
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Abstract: The Arabidopsis WUSCHEL (WUS) protein, which plays an important role in the specification of the stem cells in the shoot apical meristem (SAM), contains an 'atypical' homeodomain (HD) with extra residues in its loop and turn regions. We speculated that a WUS-type atypical HD protein might also be involved in the specification and maintenance of the root apical meristem (RAM) stem cells of rice. To investigate this possibility, we isolated and characterized a rice WUS-type homeobox gene designated quiescent-center-specific homeobox (QHB) gene. Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root. During embryogenesis and crown root formation, QHB expression was observed prior to the morphological differentiation of the root. However, we detected different QHB expression patterns in the process of the RAM development, specifically between radicle and crown root formation, suggesting that the cell-fate determination of the QC may be controlled by different mechanisms. We also produced transformants that overexpress QHB or Arabidopsis WUS. These transformants did not form crown roots, but developed multiple shoots from ectopic SAMs with malformed leaves. On the basis of these observations, we propose that the WUS-type homeobox gene is involved in the specification and maintenance of the stem cells (QC cells) in the RAM, by a mechanism similar to that for WUS in the SAM. |
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| 掲載誌 | Plant J. | |
| Country | Japan | |
| 刊数 | 35 (4) | |
| ページ | 429-441 | |
| 発刊年 | 2003 | |
| PubMed ID | 12904206 | |
| PubMed Central ID | - | |
| DOI | 10.1046/j.1365-313X.2003.01816.x | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | QHB | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
