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| 著者 | Sallaud, C., M. Lorieux, E. Roumen, D Tharreau, R. Berruyer, P. Svestasrani, O. Garsmeur, A. Ghesquiere, J.L. Nottenghem | |
| タイトル | Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. | |
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Abstract: Rice progenies used for the construction of genetic maps permit exhaustive identification and characterization of resistance genes present in their parental cultivars. We inoculated a rice progeny derived from the cross IR64 x Azucena with different Magnaporthe grisea isolates that showed differential responses on the parental cultivars. By QTL mapping, nine unlinked loci conferring resistance to each isolate were identified and named Pi-24( t) to Pi-32( t). They could correspond to nine specific resistance genes. Five of these resistance loci (RLs) were mapped at chromosomal locations where no resistance gene was previously reported, defining new resistance genes. Using degenerate primers of the NBS (nucleotide binding site) motif found in many resistance genes, two resistance gene analogues (RGAs) IR86 and IR14 were identified and mapped closely to two blast RLs (resistance identified in this study, i.e. Pi-29(t) and Pi-30(t) respectively). These two RLs may correspond to the Pi-11 and Pi-a blast resistance genes previously identified. Moreover, the ir86 and ir14 genes have been identified "in silico" on the indica rice cultivar 93-11, recently sequenced by Chinese researchers. Both genes encodes NBS-LRR-like proteins that are characteristics of plant-disease resistance genes. |
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| 掲載誌 | Theor. Appl. Genet. | |
| Country | French Republic | |
| 刊数 | 106 | |
| ページ | 794-803 | |
| 発刊年 | 2003 | |
| PubMed ID | 12647052 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | PI PI1 PI10 PI11 PI12 PI157 PI17 PI18 PI21 PI22 PI23 PI24 PI25 PI26 PI27 PI28 PI29 PI30 PI31 PI32 PI33 PI6 PI7 PI8 PI9 PIA PIB PIF PII PII2 PIK PIKUR1 PILM2 PIM PIQ1 PISH PIT PITA PITQ5 PITQ6 PIZ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
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| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
