Gene - Detail

Detail of Gene

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Basic Information
CGSNL Gene Symbol ISA1
Gene Symbol Synonym OsISA1, ISA, OsISA, PHS8, PHS8/ISA1, ISA I, OsISA I, ISAI, OsISAI
Gene Name Synonym isoamylase 1, isoamylase1, sugary-1, pre-harvest sprouting 8
Protein Name ISOAMYLASE 1
Allele sugary1, isa1, sug-1, phs8, phs8-1
Chromosome No. 8
Explanation AB015615, AB093426. OsISA1 was strongly expressed in developing endosperm at the early milking stage, whereas it was present at lower expression levels in green leaves. HQ678910-HQ679078 (O. sativa and other wild rice species). GQ150881. The starch mutant sugary contains reduced activities of isoamylase1 (ISA1) (Kawagoe et al. 2005). The sugary-h (sug-h) mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1) and Starch branching enzyme IIa (OsBEIIa) (Lee et al. 2017). The sugary endosperm phenotype could be caused by a point mutation on OsISA1, which was shared by both sug-1 and sug-h mutants (Lee et al. 2017). TO:0000975: grain width. GO:1901698: response to nitrogen compound.
Trait Class Biochemical character
Seed - Morphological traits - Endosperm
Seed - Morphological traits - Grain shape
Seed - Physiological traits - Dormancy
Seed - Physiological traits - Storage substances
cDNA Accession No. AB015615
MSU ID LOC_Os08g40930.1
RAP ID Os08g0520900
Links Oryzabase Chromosome View ( IRGSP 1.0 / Build5 )
RAP-DB ( IRGSP 1.0 / Build5 )
Related IDs List ( IRGSP 1.0 / Build5 )
INSD Accession List
(Test version)
Link to INSD Accession List
Link map Classical linkage map
Liu X., Luo J., Li T., Yang H., Wang P., Su L., Zheng Y., Bao C., Zhou C.
Rice (N Y) 2021  14(1)  25
SDG711 Is Involved in Rice Seed Development through Regulation of Starch Metabolism Gene Expression in Coordination with Other Histone Modifications.
Ali I., Ullah S., Iqbal A., Quan Z., Liang H., Ahmad S., Muhammad I., Amanullah, Imran, Guo Z., Wei S., Jiang L.
BMC Plant Biol. 2021  21(1)  600
Combined application of biochar and nitrogen fertilizer promotes the activity of starch metabolism enzymes and the expression of related genes in rice in a dual cropping system.
Baysal C., He W., Drapal M., Villorbina G., Medina V., Capell T., Khush G.S., Zhu C., Fraser P.D., Christou P.
Proc. Natl. Acad. Sci. U.S.A. 2020  117(42)  26503-26512
Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming.
Xue M., Liu L., Yu Y., Zhu J., Gao H., Wang Y., Wan J.
Rice (N Y) 2019  12(1)  100
Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes.
Long W., Wang Y., Zhu S., Jing W., Wang Y., Ren Y., Tian Y., Liu S., Liu X., Chen L., Wang D., Zhong M., Zhang Y., Hu T., Zhu J., Hao Y., Zhu X., Zhang W., Wang C., Zhang W., Wan J.
Plant Physiol. 2018  177(2)  698-712
FLOURY shrunken endosperm1 Connects Phospholipid Metabolism and Amyloplast Development in Rice.
Du L., Xu F., Fang J., Gao S., Tang J., Fang S., Wang H., Tong H., Zhang F., Chu J., Wang G., Chu C.
Plant J. 2018  95(3)  545-556
Endosperm sugar accumulation caused by mutation of phs8/ISA1 leads to pre-harvest sprouting in rice.
Lee Y., Choi M.S., Lee G., Jang S., Yoon M.R., Kim B., Piao R., Woo M.O., Chin J.H., Koh H.J.
Rice (N Y) 2017  10(1)  33
sugary endosperm is Modulated by Starch branching enzyme IIa in Rice (Oryza sativa L.).
Wu Y.P., Pu C.H., Lin H.Y., Huang H.Y., Huang Y.C., Hong C.Y., Chang M.C., Lin Y.R.
Plant Sci. 2015  233  44-52
Three novel alleles of FLOURY ENDOSPERM2 (flo2) confer dull grains with low amylose content in rice.
Su J., Hu C., Yan X., Jin Y., Chen Z., Guan Q., Wang Y., Zhong D., Jansson C., Wang F., Schnürer A., Sun C.
Nature 2015  523(7562)  602-6
Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice.
Peng C., Wang Y., Liu F., Ren Y., Zhou K., Lv J., Zheng M., Zhao S., Zhang L., Wang C., Jiang L., Zhang X., Guo X., Bao Y., Wan J.
Plant J. 2014  77(6)  917-30
FLOURY ENDOSPERM6 encodes a CBM48 domain-containing protein involved in compound granule formation and starch synthesis in rice endosperm.
Lu F.H., Park Y.J.
J. Cereal Sci. 2012  56  706-712
An SNP downstream of the OsBEIIb gene is significantly associated with amylose content and viscosity properties in rice (Oryza sativa L.)
Yu,G., Olsen,K.M. and Schaal,B.A.
Mol. Biol. Evol. 2011  28(1)  659-671
Molecular Evolution of the Endosperm Starch Synthesis Pathway Genes in Rice (Oryza sativa L.) and Its Wild Ancestor, O. rufipogon L
Utsumi Y. , Utsumi C. , Sawada T. , Fujita N. , Nakamura Y.
Plant Physiol. 2011  156(1)  61-77
Functional diversity of isoamylase oligomers: the ISA1 homo-oligomer is essential for amylopectin biosynthesis in rice endosperm.
Yun M.S. , Umemoto T. , Kawagoe Y.
Plant Cell Physiol. 2011  52(6)  1068-82
Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis.
Yan CJ,Tian ZX,Fang YW,Yang YC,Li J,Zeng SY,Gu SL,Xu CW,Tang SZ,Gu MH
Theor. Appl. Genet. 2011  122(1)  63-76
Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.).
Tian,Z., Qian,Q., Liu,Q., Yan,M., Liu,X., Yan,C., Liu,G., Gao,Z., Tang,S., Zeng,D., Wang,Y., Yu,J., Gu,M. and Li,J.
Proc. Natl. Acad. Sci. U.S.A. 2009  106(51)  21760-5
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities
Kubo A, Rahman S, Utsumi Y, Li Z, Mukai Y, Yamamoto M, Ugaki M, Harada K, Satoh H, Konik-Rose C, Morell M, Nakamura Y.
Plant Physiol. 2005  137(1)  43-56
Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.
Kawagoe Y., Kubo A., Satoh H., Takaiwa F., Nakamura Y.
Plant J. 2005  42(2)  164-74
Roles of isoamylase and ADP-glucose pyrophosphorylase in starch granule synthesis in rice endosperm.
Rahman,S., Nakamura,Y., Li,Z., Clarke,B., Fujita,N., Mukai,Y., Yamamoto,M., Regina,A., Tan,Z., Kawasaki,S. and Morell,M.
Genome 2003  46(3)  496-506
The sugary-type isoamylase gene from rice and Aegilops tauschii: characterization and comparison with maize and Arabidopsis
Kubo A., Fujita N., Harada K., Matsuda T., Satoh H., Nakamura Y.
Plant Physiol. 1999  121(2)  399-410
The starch-debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm
Fujita,N., Kubo,A., Francisco,P.B.,Jr., Nakakita,M., Harada,K., Minaka,N. and Nakamura,Y.
Planta. 1999  208(2)  283-293
Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice.
TextPresso Search Search textpresso for ISA1 ( Recent references may be retrievable, but without any warranty )
DB Reference
Gramene ID -
Gene Ontology cation binding( GO:0043169 )
isoamylase activity( GO:0019156 )
endosperm development( GO:0009960 )
starch biosynthetic process( GO:0019252 )
regulation of seed germination( GO:0010029 )
maintenance of seed dormancy( GO:0010231 )
glycogen catabolic process( GO:0005980 )
abscisic acid mediated signaling( GO:0009738 )
response to abscisic acid stimulus( GO:0009737 )
seed germination on parent plant( GO:0048623 )
Trait Ontology sugary endosperm( TO:0000099 )
starch grain synthesis( TO:0002658 )
grain length( TO:0000734 )
grain thickness( TO:0000399 )
abscisic acid sensitivity( TO:0000615 )
nitrogen sensitivity( TO:0000011 )
Plant Ontology seed maturation stage( PO:0007632 )
Related Strains
Phenotype images
Last updated
Jun 6, 2022