|
Basic Information
|
|
CGSNL Gene Symbol
|
PHS8
|
|
Gene Symbol Synonym
|
ISA1, OsISA1, ISA, OsISA, PHS8/ISA1, OsPHS8, ISA I, OsISA I, ISAI, OsISAI
|
|
CGSNL Gene Name
|
PRE-HARVEST SPROUTING 8
|
|
Gene Name Synonym
|
isoamylase 1, isoamylase1, sugary-1, pre-harvest sprouting 8
|
|
Protein Name
|
ISOAMYLASE 1
|
|
Allele
|
sugary1, isa1, sug-1, phs8, phs8-1
|
|
Chromosome No.
|
8
|
|
Explanation
|
AB015615, AB093426. OsISA1 was strongly expressed in developing endosperm at the early milking stage, whereas it was present at lower expression levels in green leaves. HQ678910-HQ679078 (O. sativa and other wild rice species). GQ150881. The starch mutant sugary contains reduced activities of isoamylase1 (ISA1) (Kawagoe et al. 2005). The sugary-h (sug-h) mutant phenotype was controlled by a complementary interaction of two recessive genes, Isoamylase1 (OsISA1) and Starch branching enzyme IIa (OsBEIIa) (Lee et al. 2017). The sugary endosperm phenotype could be caused by a point mutation on OsISA1, which was shared by both sug-1 and sug-h mutants (Lee et al. 2017). TO:0000975: grain width. GO:1901698: response to nitrogen compound.
|
|
Trait Class
|
Biochemical character
Seed - Morphological traits - Endosperm
Seed - Morphological traits - Grain shape
Seed - Physiological traits - Dormancy
Seed - Physiological traits - Storage substances
Tolerance and resistance - Stress tolerance
|
|
Expression
|
|
|
Sequence/Locus
|
|
cDNA Accession No.
|
AB015615
|
|
MSU ID
|
LOC_Os08g40930.1
|
|
RAP ID
|
Os08g0520900
|
|
Links
|
Oryzabase Chromosome View
(
IRGSP 1.0
/
Build5
)
RAP-DB
(
IRGSP 1.0
/
Build5
)
Related IDs List (
IRGSP 1.0
/
Build5
)
|
INSD Accession List
(Test version)
|
Link to INSD Accession List
|
|
Map
|
|
Locate(cM)
|
|
|
Link map
|
Classical linkage map
|
|
References
|
|
Yan H., Zhang W., Wang Y., Jin J., Xu H., Fu Y., Shan Z., Wang X., Teng X., Li X., Wang Y., Hu X., Zhang W., Zhu C., Zhang X., Zhang Y., Wang R., Zhang J., Cai Y., You X., Chen J., Ge X., Wang L., Xu J., Jiang L., Liu S., Lei C., Zhang X., Wang H., Ren Y., Wan J.
Plant Cell 2024
Rice LIKE EARLY STARVATION1 cooperates with floury endosperm 6 to modulate starch biosynthesis and endosperm development.
|
|
Kamble N.U.
Plant Cell 2024 36(5) 1590-1591
Directing a colleague: LIKE EARLY STARVATION with FLOURY ENDOSPERM 6 targets isoamylase 1 to rice starch granules.
|
|
Guo D., Chen L., Liu S., Jiang W., Ye Q., Wu Z., Wang X., Hu X., Zhang Z., He H., Hu L.
Plants (Basel) 2023 12(17)
<i>Curling Leaf 1</i>, Encoding a MYB-Domain Protein, Regulates Leaf Morphology and Affects Plant Yield in Rice.
|
|
Teng Z., Zheng Q., Liu B., Meng S., Zhang J., Ye N.
Int J Mol Sci 2022 23(14)
Moderate Soil Drying-Induced Alternative Splicing Provides a Potential Novel Approach for the Regulation of Grain Filling in Rice Inferior Spikelets.
|
|
Ali I., Ullah S., Iqbal A., Quan Z., Liang H., Ahmad S., Muhammad I., Amanullah, Imran, Guo Z., Wei S., Jiang L.
BMC Plant Biol. 2021 21(1) 600
Combined application of biochar and nitrogen fertilizer promotes the activity of starch metabolism enzymes and the expression of related genes in rice in a dual cropping system.
|
|
Liu X., Luo J., Li T., Yang H., Wang P., Su L., Zheng Y., Bao C., Zhou C.
Rice (N Y) 2021 14(1) 25
SDG711 Is Involved in Rice Seed Development through Regulation of Starch Metabolism Gene Expression in Coordination with Other Histone Modifications.
|
|
Baysal C., He W., Drapal M., Villorbina G., Medina V., Capell T., Khush G.S., Zhu C., Fraser P.D., Christou P.
Proc. Natl. Acad. Sci. U.S.A. 2020 117(42) 26503-26512
Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming.
|
|
Xue M., Liu L., Yu Y., Zhu J., Gao H., Wang Y., Wan J.
Rice (N Y) 2019 12(1) 100
Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes.
|
|
Long W., Wang Y., Zhu S., Jing W., Wang Y., Ren Y., Tian Y., Liu S., Liu X., Chen L., Wang D., Zhong M., Zhang Y., Hu T., Zhu J., Hao Y., Zhu X., Zhang W., Wang C., Zhang W., Wan J.
Plant Physiol. 2018 177(2) 698-712
FLOURY shrunken endosperm1 Connects Phospholipid Metabolism and Amyloplast Development in Rice.
|
|
Du L., Xu F., Fang J., Gao S., Tang J., Fang S., Wang H., Tong H., Zhang F., Chu J., Wang G., Chu C.
Plant J. 2018 95(3) 545-556
Endosperm sugar accumulation caused by mutation of phs8/ISA1 leads to pre-harvest sprouting in rice.
|
|
Lee Y., Choi M.S., Lee G., Jang S., Yoon M.R., Kim B., Piao R., Woo M.O., Chin J.H., Koh H.J.
Rice (N Y) 2017 10(1) 33
sugary endosperm is Modulated by Starch branching enzyme IIa in Rice (Oryza sativa L.).
|
|
Su J., Hu C., Yan X., Jin Y., Chen Z., Guan Q., Wang Y., Zhong D., Jansson C., Wang F., Schnürer A., Sun C.
Nature 2015 523(7562) 602-6
Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice.
|
|
Wu Y.P., Pu C.H., Lin H.Y., Huang H.Y., Huang Y.C., Hong C.Y., Chang M.C., Lin Y.R.
Plant Sci. 2015 233 44-52
Three novel alleles of FLOURY ENDOSPERM2 (flo2) confer dull grains with low amylose content in rice.
|
|
Peng C., Wang Y., Liu F., Ren Y., Zhou K., Lv J., Zheng M., Zhao S., Zhang L., Wang C., Jiang L., Zhang X., Guo X., Bao Y., Wan J.
Plant J. 2014 77(6) 917-30
FLOURY ENDOSPERM6 encodes a CBM48 domain-containing protein involved in compound granule formation and starch synthesis in rice endosperm.
|
|
Lu F.H., Park Y.J.
J. Cereal Sci. 2012 56 706-712
An SNP downstream of the OsBEIIb gene is significantly associated with amylose content and viscosity properties in rice (Oryza sativa L.)
|
|
Yun M.S. , Umemoto T. , Kawagoe Y.
Plant Cell Physiol. 2011 52(6) 1068-82
Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis.
|
|
Yu,G., Olsen,K.M. and Schaal,B.A.
Mol. Biol. Evol. 2011 28(1) 659-671
Molecular Evolution of the Endosperm Starch Synthesis Pathway Genes in Rice (Oryza sativa L.) and Its Wild Ancestor, O. rufipogon L
|
|
Yan CJ,Tian ZX,Fang YW,Yang YC,Li J,Zeng SY,Gu SL,Xu CW,Tang SZ,Gu MH
Theor. Appl. Genet. 2011 122(1) 63-76
Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.).
|
|
Utsumi Y. , Utsumi C. , Sawada T. , Fujita N. , Nakamura Y.
Plant Physiol. 2011 156(1) 61-77
Functional diversity of isoamylase oligomers: the ISA1 homo-oligomer is essential for amylopectin biosynthesis in rice endosperm.
|
|
Tian,Z., Qian,Q., Liu,Q., Yan,M., Liu,X., Yan,C., Liu,G., Gao,Z., Tang,S., Zeng,D., Wang,Y., Yu,J., Gu,M. and Li,J.
Proc. Natl. Acad. Sci. U.S.A. 2009 106(51) 21760-5
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities
|
|
Kubo A, Rahman S, Utsumi Y, Li Z, Mukai Y, Yamamoto M, Ugaki M, Harada K, Satoh H, Konik-Rose C, Morell M, Nakamura Y.
Plant Physiol. 2005 137(1) 43-56
Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.
|
|
Kawagoe Y., Kubo A., Satoh H., Takaiwa F., Nakamura Y.
Plant J. 2005 42(2) 164-74
Roles of isoamylase and ADP-glucose pyrophosphorylase in starch granule synthesis in rice endosperm.
|
|
Rahman,S., Nakamura,Y., Li,Z., Clarke,B., Fujita,N., Mukai,Y., Yamamoto,M., Regina,A., Tan,Z., Kawasaki,S. and Morell,M.
Genome 2003 46(3) 496-506
The sugary-type isoamylase gene from rice and Aegilops tauschii: characterization and comparison with maize and Arabidopsis
|
|
Kubo A., Fujita N., Harada K., Matsuda T., Satoh H., Nakamura Y.
Plant Physiol. 1999 121(2) 399-410
The starch-debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm
|
|
Fujita,N., Kubo,A., Francisco,P.B.,Jr., Nakakita,M., Harada,K., Minaka,N. and Nakamura,Y.
Planta. 1999 208(2) 283-293
Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice.
|
|
TextPresso Search
|
Search textpresso for PHS8
( Recent references may be retrievable, but without any warranty )
|
|
DB Reference
|
|
Gramene ID
|
-
|
|
Ontologies
|
|
Gene Ontology
|
regulation of starch biosynthetic process( GO:0010581 )
abscisic acid mediated signaling( GO:0009738 )
glycogen catabolic process( GO:0005980 )
response to desiccation( GO:0009269 )
response to abscisic acid stimulus( GO:0009737 )
cation binding( GO:0043169 )
isoamylase activity( GO:0019156 )
endosperm development( GO:0009960 )
starch biosynthetic process( GO:0019252 )
regulation of seed germination( GO:0010029 )
maintenance of seed dormancy( GO:0010231 )
seed germination on parent plant( GO:0048623 )
|
|
Trait Ontology
|
sugary endosperm( TO:0000099 )
starch grain synthesis( TO:0002658 )
grain length( TO:0000734 )
grain thickness( TO:0000399 )
abscisic acid sensitivity( TO:0000615 )
nitrogen sensitivity( TO:0000011 )
drought related trait( TO:0000394 )
|
|
Plant Ontology
|
seed maturation stage( PO:0007632 )
endosperm development stage( PO:0007633 )
|
|
Related Strains
|
|
-
|
|
Phenotype images
|
|
-
|
|
Last updated
|
|
Aug 28, 2024
|
Update info.
Click here for feedback