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Basic Information
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CGSNL Gene Symbol
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NAS2
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Gene Symbol Synonym
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OsNAS2, OsNAS1
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CGSNL Gene Name
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NICOTIANAMINE SYNTHASE 2
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Gene Name Synonym
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rice nicotianamine synthase2, NA synthase gene 2, Nicotianamine synthase 2, S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2, NA synthase 2
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Protein Name
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NICOTIANAMINE SYNTHASE 2
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Allele
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Chromosome No.
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3
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Explanation
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The nicotianamine is a crucial component of the iron acquisition apparatus of graminaceous plants. EC=2.5.1.43 A2XFU5(indica). Q10MI9(japonica). AU176507. OsNAS1 in Sanchez-Sanuy et al. 2019. TO:0006049: iron concentration. TO:0020089: iron content trait. TO:0006053: zinc concentration. TO:0020090: zinc content trait. GO:1990641: response to iron ion starvation. GO:0071579: regulation of zinc ion transport. TO:0001072: inflorescence axis length. TO:0020091: manganese content. TO:0020092: copper content trait. TO:0006044: magnesium content. TO:0000975: grain width.
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Trait Class
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Biochemical character
Vegetative organ - Culm
Reproductive organ - panicle
Seed - Morphological traits - Grain shape
Seed - Physiological traits - Storage substances
Tolerance and resistance - Stress tolerance
Character as QTL - Yield and productivity
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Expression
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Sequence/Locus
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cDNA Accession No.
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AK112011
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MSU ID
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LOC_Os03g19420.2
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RAP ID
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Os03g0307200
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Links
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Oryzabase Chromosome View
(
IRGSP 1.0
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Build5
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RAP-DB
(
IRGSP 1.0
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Build5
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Related IDs List (
IRGSP 1.0
/
Build5
)
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INSD Accession List
(Test version)
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Link to INSD Accession List
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Map
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Locate(cM)
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Link map
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Classical linkage map
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References
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Rahman M.M., Seo D.Y., Rahman M.M., Huang C.Y., Ogawa A., Beaurepère T., Aung M.S., Ouerdane L., Song W.Y., Lee S., An G., Jeon J.S.
J. Agric. Food Chem. 2025 73(38) 24262-24277
Toward Nutrient-Rich Rice: Biofortification through Mineral Accumulation and Low Phytic Acid Content.
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Wang W., He F., Zhang H., Yang Y., Wang X., Fu Y., Shou H., Zheng L.
Abiotech 2025 6(2) 215-231
OsbHLH062 regulates iron homeostasis by inhibiting iron deficiency responses in rice.
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Zhu J., Li J., Hu X., Wang J., Fang J., Wang S., Shou H.
J. Exp. Bot. 2024 75(3) 1112-1127
Role of transcription factor complex OsbHLH156-OsIRO2 in regulating manganese, copper, and zinc transporters in rice.
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Ludwig Y., Dueñas C., Arcillas E., Macalalad-Cabral RJ., Kohli A., Reinke R., Slamet-Loedin IH.
Front Genome Ed 2023 5 1308228
CRISPR-mediated promoter editing of a <i>cis</i>-regulatory element of <i>OsNAS2</i> increases Zn uptake/translocation and plant yield in rice.
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Chowdhury R., Nallusamy S., Shanmugam V., Loganathan A., Muthurajan R., Subramanian Kizhaeral Sivathapandian, Paramasivam J., Duraialagaraja S.
Biologia 2022 77 39-53
Genome‐wide understanding of evolutionary and functional relationships of rice Yellow Stripe‐Like (YSL) transporter family in comparison with other plant species
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Wang W., Shinwari K.I., Zhang H., Zhang H., Dong L., He F., Zheng L.
Int J Mol Sci 2022 23(23)
The bHLH Transcription Factor OsbHLH057 Regulates Iron Homeostasis in Rice.
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Cao L., Gao Y., Yu J., Niu S., Zeng J., Yao Q., Wang X., Bu Z., Xu T., Liu X., Zhu Y.
Plant Physiol. Biochem. 2021 158 275-283
Streptomyces hygroscopicus OsiSh-2-induced mitigation of Fe deficiency in rice plants.
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Wang S., Li L., Ying Y., Wang J., Shao J.F., Yamaji N., Whelan J., Ma J.F., Shou H.
New Phytol. 2020 225(3) 1247-1260
A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice.
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Yang A., Li Q., Chen L., Zhang W.H.
J. Exp. Bot. 2020
A rice small GTPase, OsRab6a, is involved in the regulation of grain yield and iron nutrition in response to CO2 enrichment.
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Liang G., Zhang H., Li Y., Pu M., Yang Y., Li C., Lu C., Xu P., Yu D.
J Integr Plant Biol 2020 62(5) 668-689
Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis.
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Sánchez-Sanuy F., Peris-Peris C., Tomiyama S., Okada K., Hsing Y.I., San Segundo B., Campo S.
BMC Plant Biol. 2019 19(1) 563
Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus.
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Aung M.S., Kobayashi T., Masuda H., Nishizawa N.K.
Physiol Plant 2018
Rice HRZ ubiquitin ligases are crucial for response to excess iron.
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Masuda H., Shimochi E., Hamada T., Senoura T., Kobayashi T., Aung M.S., Ishimaru Y., Ogo Y., Nakanishi H., Nishizawa N.K.
PLoS ONE 2017 12(3) e0173441
A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil.
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Wang B., Wei H., Xue Z., Zhang W.H.
Ann. Bot. 2017 119(6) 945-956
Gibberellins regulate iron deficiency-response by influencing iron transport and translocation in rice seedlings (Oryza sativa).
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Andrés-Bordería A., Andrés F., Garcia-Molina A., Perea-García A., Domingo C., Puig S., Peñarrubia L.
Plant Mol. Biol. 2017
Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.).
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Zhang H., Li Y., Yao X., Liang G., Yu D.
Plant Physiol. 2017 175(1) 543-554
POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis.
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Tan S., Liu F., Pan X.X., Zang Y.P., Jin F., Zu W.X., Qi X.T., Xiao W., Yin L.P.
Sci Rep 2016 6 25485
CSN6, a subunit of the COP9 signalosome, is involved in early response to iron deficiency in Oryza sativa.
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Kobayashi T., Itai R.N., Senoura T., Oikawa T., Ishimaru Y., Ueda M., Nakanishi H., Nishizawa N.K.
Plant Mol. Biol. 2016 91(4-5) 533-47
Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.
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Moreno-Moyano LT., Bonneau J.P., Sánchez-Palacios JT., Tohme J., Johnson A.A.
Front Plant Sci 2016 7 1463
Association of Increased Grain Iron and Zinc Concentrations with Agro-morphological Traits of Biofortified Rice.
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Yang A., Zhang W.H.
Plant Cell Physiol. 2016 57(6) 1271-80
A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice.
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Bashir K., Ishimaru Y., Itai R.N., Senoura T., Takahashi M., An G., Oikawa T., Ueda M., Sato A., Uozumi N., Nakanishi H., Nishizawa N.K.
Plant Mol. Biol. 2015 88(1-2) 165-76
Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice.
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Hu B., Wang W., Deng K., Li H., Zhang Z., Zhang L., Chu C.
Front Plant Sci 2015 6 188
MicroRNA399 is involved in multiple nutrient starvation responses in rice.
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Nozoye T., Tsunoda K., Nagasaka S., Bashir K., Takahashi M., Kobayashi T., Nakanishi H., Nishizawa N.K.
Plant Signal Behav 2014 9
Rice nicotianamine synthase localizes to particular vesicles for proper function.
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Agarwal S., Tripura Venkata VG., Kotla A., Mangrauthia S.K., Neelamraju S.
Gene 2014 546(2) 430-6
Expression patterns of QTL based and other candidate genes in Madhukar × Swarna RILs with contrasting levels of iron and zinc in unpolished rice grains.
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Kobayashi T.a b, Nakanishi Itai R.c, Nishizawa N.K.b
Rice 2014 7
Iron deficiency responses in rice roots
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Nozoye T.a, Nagasaka S.a c, Bashir K.a d, Takahashi M.a e, Kobayashi T.b, Nakanishi H.a, Nishizawa N.K.a b
Plant Journal 2014 77 246-260
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXphi or LL motif causes the disruption of vesicle formation or movement in rice
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Yang A., Li Y., Xu Y., Zhang W.H.
J. Exp. Bot. 2013 64(16) 5009-20
A receptor-like protein RMC is involved in regulation of iron acquisition in rice.
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Nozoye T., Nagasaka S., Bashir K., Takahashi M., Kobayashi T., Nakanishi H., Nishizawa N.K.
Plant J. 2013
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXphi or LL motif causes the disruption of vesicle formation or movement in rice.
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Lee S., Kim Y.S., Jeon U.S., Kim Y.K., Schjoerring J.K., An G.
Mol. Cells 2012 33(3) 269-75
Activation of Rice nicotianamine synthase 2 (OsNAS2) enhances iron availability for biofortification.
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Anuradha K., Agarwal S., Rao Y.V., Rao K.V., Viraktamath B.C., Sarla N.
Gene 2012 508(2) 233-40
Mapping QTLs and candidate genes for iron and zinc concentrations in unpolished rice of Madhukar×Swarna RILs.
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Suzuki M., Bashir K., Inoue H., Takahashi M., Nakanishi H., Nishizawa N.K.
Rice (N Y) 2012 5 9
Accumulation of starch in Zn-deficient rice
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Wang L., Ying Y., Narsai R., Ye L., Zheng L., Tian J., Whelan J., Shou H.
Plant Cell Environ. 2012 36(1) 224-36.
Identification of OsbHLH133 as a regulator of iron distribution between roots and shoots in Oryza sativa.
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Johnson A.A., Kyriacou B., Callahan D.L., Carruthers L., Stangoulis J., Lombi E., Tester M.
PLoS ONE 2011 6(9) e24476
Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm.
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Ishimaru Y., Masuda H., Bashir K., Inoue H., Tsukamoto T., Takahashi M., Nakanishi H., Aoki N., Hirose T., Ohsugi R., Naoko K Nishizawa
Plant J. 2010 62(3) 379-90
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.
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Kobayashi T,Itai RN,Ogo Y,Kakei Y,Nakanishi H,Takahashi M,Nishizawa NK
Plant J. 2009 60(6) 948-61
The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes.
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Narayanan NN, Vasconcelos MW, Grusak MA.
Plant Physiol. Biochem. 2007 45(5) 277-86.
Expression profiling of Oryza sativa metal homeostasis genes in different rice cultivars using a cDNA macroarray.
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Kobayashi T,Ogo Y,Itai RN,Nakanishi H,Takahashi M,Mori S,Nishizawa NK
Proc. Natl. Acad. Sci. U.S.A. 2007 104 19150-5
The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants.
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Kobayashi,T., Suzuki,M., Inoue,H., Itai,R.N., Takahashi,M., Nakanishi,H., Mori,S. and Nishizawa,N.K.
J. Exp. Bot. 2005 56 1305-1316
Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements
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Nozoye T., Reiko Nakanishi Ita, Nagasaki S., Takahashi M., Nakanishi H., Mori S., Naoko K Nishikawa
Soil Sci Plant Nutr 2004 50(7) 1125-1131
Diurnal Changes in the Expression of Genes That Participate in Phytosiderophore Synthesis in Rice
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Inoue, H., K. Higuchi, M. Takahashi, H. Nakanishi, S. Mori and N.K. Nishizawa
Plant J. 2003 36 (3) 366-381
Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron.
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Higuchi,K., Watanabe,S., Takahashi,M., Kawasaki,S., Nakanishi,H., Nishizawa,N.K. and Mori,S.
Plant J. 2001 25(2) 159-167
Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions.
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Higuchi K, Suzuki K, Nakanishi H, Yamaguchi H, Nishizawa NK, Mori S.
Plant Physiol. 1999 119(2) 471-480
Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores.
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DB Reference
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Gramene ID
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GR:0080048
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Ontologies
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Gene Ontology
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zinc ion transport( GO:0006829 )
response to gibberellin stimulus( GO:0009739 )
response to symbiotic bacterium( GO:0009609 )
response to starvation( GO:0042594 )
iron ion transport( GO:0006826 )
nicotianamine synthase activity( GO:0030410 )
nicotianamine biosynthetic process( GO:0030418 )
vesicle-mediated transport( GO:0016192 )
iron ion homeostasis( GO:0055072 )
cellular response to iron ion starvation( GO:0010106 )
response to iron ion( GO:0010039 )
response to copper ion( GO:0046688 )
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Trait Ontology
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iron sensitivity( TO:0000224 )
chlorophyll content( TO:0000495 )
gibberellic acid sensitivity( TO:0000166 )
copper sensitivity( TO:0000021 )
grain yield( TO:0000396 )
spikelet number( TO:0000456 )
grain number per plant( TO:0000440 )
grain weight( TO:0000590 )
leaf width( TO:0000370 )
panicle length( TO:0000040 )
filled grain number( TO:0000447 )
plant height( TO:0000207 )
panicle number( TO:0000152 )
100-seed weight( TO:0000269 )
mineral and ion content related trait( TO:0000465 )
potassium content( TO:0000609 )
grain length( TO:0000734 )
grain width( TO:0000402 )
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Plant Ontology
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vascular leaf( PO:0009025 )
seed( PO:0009010 )
shoot system( PO:0009006 )
pericycle( PO:0006203 )
root epidermis( PO:0006036 )
exodermis( PO:0005772 )
root stele( PO:0020124 )
lateral root( PO:0020121 )
root( PO:0009005 )
root cortex( PO:0000258 )
protoxylem( PO:0000272 )
vascular bundle( PO:0005020 )
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Related Strains
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Phenotype images
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Last updated
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Feb 18, 2026
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Update info.