Gene - Detail

Detail of Gene

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Basic Information
CGSNL Gene Symbol NAS2
Gene Symbol Synonym OsNAS2, OsNAS1
Gene Name Synonym rice nicotianamine synthase2, NA synthase gene 2, Nicotianamine synthase 2, S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2, NA synthase 2
Chromosome No. 3
Explanation The nicotianamine is a crucial component of the iron acquisition apparatus of graminaceous plants. EC= A2XFU5(indica). Q10MI9(japonica). AU176507. OsNAS1 in Sanchez-Sanuy et al. 2019. TO:0006049: iron concentration. TO:0006053: zinc concentration. GO:1990641: response to iron ion starvation. GO:0071579: regulation of zinc ion transport. TO:0001072: inflorescence axis length.
Trait Class Biochemical character
Vegetative organ - Culm
Reproductive organ - panicle
Tolerance and resistance - Stress tolerance
Character as QTL - Yield and productivity
cDNA Accession No. AK112011
MSU ID LOC_Os03g19420.2
RAP ID Os03g0307200
Links Oryzabase Chromosome View ( IRGSP 1.0 / Build5 )
RAP-DB ( IRGSP 1.0 / Build5 )
Related IDs List ( IRGSP 1.0 / Build5 )
INSD Accession List
(Test version)
Link to INSD Accession List
Link map Classical linkage map
Ludwig Y., Dueñas C., Arcillas E., Macalalad-Cabral RJ., Kohli A., Reinke R., Slamet-Loedin IH.
Front Genome Ed 2023  5  1308228
CRISPR-mediated promoter editing of a <i>cis</i>-regulatory element of <i>OsNAS2</i> increases Zn uptake/translocation and plant yield in rice.
Wang W., Shinwari K.I., Zhang H., Zhang H., Dong L., He F., Zheng L.
Int J Mol Sci 2022  23(23) 
The bHLH Transcription Factor OsbHLH057 Regulates Iron Homeostasis in Rice.
Cao L., Gao Y., Yu J., Niu S., Zeng J., Yao Q., Wang X., Bu Z., Xu T., Liu X., Zhu Y.
Plant Physiol. Biochem. 2021  158  275-283
Streptomyces hygroscopicus OsiSh-2-induced mitigation of Fe deficiency in rice plants.
Yang A., Li Q., Chen L., Zhang W.H.
J. Exp. Bot. 2020   
A rice small GTPase, OsRab6a, is involved in the regulation of grain yield and iron nutrition in response to CO2 enrichment.
Liang G., Zhang H., Li Y., Pu M., Yang Y., Li C., Lu C., Xu P., Yu D.
J Integr Plant Biol 2020  62(5)  668-689
Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis.
Wang S., Li L., Ying Y., Wang J., Shao J.F., Yamaji N., Whelan J., Ma J.F., Shou H.
New Phytol. 2020  225(3)  1247-1260
A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localising IRO2 to the nucleus in rice.
Sánchez-Sanuy F., Peris-Peris C., Tomiyama S., Okada K., Hsing Y.I., San Segundo B., Campo S.
BMC Plant Biol. 2019  19(1)  563
Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus.
Aung M.S., Kobayashi T., Masuda H., Nishizawa N.K.
Physiol Plant 2018   
Rice HRZ ubiquitin ligases are crucial for response to excess iron.
Masuda H., Shimochi E., Hamada T., Senoura T., Kobayashi T., Aung M.S., Ishimaru Y., Ogo Y., Nakanishi H., Nishizawa N.K.
PLoS ONE 2017  12(3)  e0173441
A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil.
Zhang H., Li Y., Yao X., Liang G., Yu D.
Plant Physiol. 2017  175(1)  543-554
Andrés-Bordería A., Andrés F., Garcia-Molina A., Perea-García A., Domingo C., Puig S., Peñarrubia L.
Plant Mol. Biol. 2017   
Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.).
Wang B., Wei H., Xue Z., Zhang W.H.
Ann. Bot. 2017  119(6)  945-956
Gibberellins regulate iron deficiency-response by influencing iron transport and translocation in rice seedlings (Oryza sativa).
Kobayashi T., Itai R.N., Senoura T., Oikawa T., Ishimaru Y., Ueda M., Nakanishi H., Nishizawa N.K.
Plant Mol. Biol. 2016  91(4-5)  533-47
Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.
Moreno-Moyano LT., Bonneau J.P., Sánchez-Palacios JT., Tohme J., Johnson A.A.
Front Plant Sci 2016  7  1463
Association of Increased Grain Iron and Zinc Concentrations with Agro-morphological Traits of Biofortified Rice.
Tan S., Liu F., Pan X.X., Zang Y.P., Jin F., Zu W.X., Qi X.T., Xiao W., Yin L.P.
Sci Rep 2016  6  25485
CSN6, a subunit of the COP9 signalosome, is involved in early response to iron deficiency in Oryza sativa.
Yang A., Zhang W.H.
Plant Cell Physiol. 2016  57(6)  1271-80
A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice.
Hu B., Wang W., Deng K., Li H., Zhang Z., Zhang L., Chu C.
Front Plant Sci 2015  6  188
MicroRNA399 is involved in multiple nutrient starvation responses in rice.
Bashir K., Ishimaru Y., Itai R.N., Senoura T., Takahashi M., An G., Oikawa T., Ueda M., Sato A., Uozumi N., Nakanishi H., Nishizawa N.K.
Plant Mol. Biol. 2015  88(1-2)  165-76
Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice.
Kobayashi T.a b, Nakanishi Itai R.c, Nishizawa N.K.b
Rice 2014  7 
Iron deficiency responses in rice roots
Agarwal S., Tripura Venkata VG., Kotla A., Mangrauthia S.K., Neelamraju S.
Gene 2014  546(2)  430-6
Expression patterns of QTL based and other candidate genes in Madhukar × Swarna RILs with contrasting levels of iron and zinc in unpolished rice grains.
Nozoye T., Tsunoda K., Nagasaka S., Bashir K., Takahashi M., Kobayashi T., Nakanishi H., Nishizawa N.K.
Plant Signal Behav 2014  9 
Rice nicotianamine synthase localizes to particular vesicles for proper function.
Nozoye T.a, Nagasaka S.a c, Bashir K.a d, Takahashi M.a e, Kobayashi T.b, Nakanishi H.a, Nishizawa N.K.a b
Plant Journal 2014  77  246-260
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXphi or LL motif causes the disruption of vesicle formation or movement in rice
Yang A., Li Y., Xu Y., Zhang W.H.
J. Exp. Bot. 2013  64(16)  5009-20
A receptor-like protein RMC is involved in regulation of iron acquisition in rice.
Nozoye T., Nagasaka S., Bashir K., Takahashi M., Kobayashi T., Nakanishi H., Nishizawa N.K.
Plant J. 2013   
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXphi or LL motif causes the disruption of vesicle formation or movement in rice.
Lee S., Kim Y.S., Jeon U.S., Kim Y.K., Schjoerring J.K., An G.
Mol. Cells 2012  33(3)  269-75
Activation of Rice nicotianamine synthase 2 (OsNAS2) enhances iron availability for biofortification.
Suzuki M., Bashir K., Inoue H., Takahashi M., Nakanishi H., Nishizawa N.K.
Rice (N Y) 2012  5  9
Accumulation of starch in Zn-deficient rice
Anuradha K., Agarwal S., Rao Y.V., Rao K.V., Viraktamath B.C., Sarla N.
Gene 2012  508(2)  233-40
Mapping QTLs and candidate genes for iron and zinc concentrations in unpolished rice of Madhukar×Swarna RILs.
Wang L., Ying Y., Narsai R., Ye L., Zheng L., Tian J., Whelan J., Shou H.
Plant Cell Environ. 2012  36(1)  224-36.
Identification of OsbHLH133 as a regulator of iron distribution between roots and shoots in Oryza sativa.
Johnson A.A., Kyriacou B., Callahan D.L., Carruthers L., Stangoulis J., Lombi E., Tester M.
PLoS ONE 2011  6(9)  e24476
Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm.
Ishimaru Y., Masuda H., Bashir K., Inoue H., Tsukamoto T., Takahashi M., Nakanishi H., Aoki N., Hirose T., Ohsugi R., Naoko K Nishizawa
Plant J. 2010  62(3)  379-90
Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese.
Kobayashi T,Itai RN,Ogo Y,Kakei Y,Nakanishi H,Takahashi M,Nishizawa NK
Plant J. 2009  60(6)  948-61
The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes.
Kobayashi T,Ogo Y,Itai RN,Nakanishi H,Takahashi M,Mori S,Nishizawa NK
Proc. Natl. Acad. Sci. U.S.A. 2007  104  19150-5
The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants.
Narayanan NN, Vasconcelos MW, Grusak MA.
Plant Physiol. Biochem. 2007  45(5)  277-86.
Expression profiling of Oryza sativa metal homeostasis genes in different rice cultivars using a cDNA macroarray.
Kobayashi,T., Suzuki,M., Inoue,H., Itai,R.N., Takahashi,M., Nakanishi,H., Mori,S. and Nishizawa,N.K.
J. Exp. Bot. 2005  56  1305-1316
Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements
Nozoye T., Reiko Nakanishi Ita, Nagasaki S., Takahashi M., Nakanishi H., Mori S., Naoko K Nishikawa
Soil Sci Plant Nutr 2004  50(7)  1125-1131
Diurnal Changes in the Expression of Genes That Participate in Phytosiderophore Synthesis in Rice
Inoue, H., K. Higuchi, M. Takahashi, H. Nakanishi, S. Mori and N.K. Nishizawa
Plant J. 2003  36 (3)  366-381
Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron.
Higuchi,K., Watanabe,S., Takahashi,M., Kawasaki,S., Nakanishi,H., Nishizawa,N.K. and Mori,S.
Plant J. 2001  25(2)  159-167
Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions.
Higuchi K, Suzuki K, Nakanishi H, Yamaguchi H, Nishizawa NK, Mori S.
Plant Physiol. 1999  119(2)  471-480
Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores.
TextPresso Search Search textpresso for NAS2 ( Recent references may be retrievable, but without any warranty )
DB Reference
Gramene ID GR:0080048
Gene Ontology zinc ion transport( GO:0006829 )
response to gibberellin stimulus( GO:0009739 )
response to symbiotic bacterium( GO:0009609 )
response to starvation( GO:0042594 )
iron ion transport( GO:0006826 )
nicotianamine synthase activity( GO:0030410 )
nicotianamine biosynthetic process( GO:0030418 )
vesicle-mediated transport( GO:0016192 )
iron ion homeostasis( GO:0055072 )
cellular response to iron ion starvation( GO:0010106 )
response to iron ion( GO:0010039 )
response to copper ion( GO:0046688 )
Trait Ontology iron sensitivity( TO:0000224 )
chlorophyll content( TO:0000495 )
gibberellic acid sensitivity( TO:0000166 )
copper sensitivity( TO:0000021 )
grain yield( TO:0000396 )
spikelet number( TO:0000456 )
grain number per plant( TO:0000440 )
grain weight( TO:0000590 )
leaf width( TO:0000370 )
panicle length( TO:0000040 )
filled grain number( TO:0000447 )
plant height( TO:0000207 )
panicle number( TO:0000152 )
100-seed weight( TO:0000269 )
Plant Ontology vascular leaf( PO:0009025 )
seed( PO:0009010 )
shoot system( PO:0009006 )
pericycle( PO:0006203 )
root epidermis( PO:0006036 )
exodermis( PO:0005772 )
root stele( PO:0020124 )
lateral root( PO:0020121 )
root( PO:0009005 )
root cortex( PO:0000258 )
protoxylem( PO:0000272 )
vascular bundle( PO:0005020 )
Related Strains
Phenotype images
Last updated
Jun 21, 2024