Faculty of Agriculture, Hirosaki University, Hirosaki, 036 Japan
Acc259, a native Japonica variety from Philippines kept in National Institute of Genetics as a tester, was found to have a rare allele, Gdh-12 for glutamate dehydrogenase. Its linkage relation was examined using this variety, and was found to be linked with Pgi-1 for phosphoglucose isomerase on chromosome 3 with a recombination value of 8.4% (Ishikawa et al. 1991). To determine the map positions of these genes, their linkage relations with morphological markers on chromosome 3 were examined.
In an F2 population from a cross between FL58 having chl-1 (chlorina-1) and Acc259, Gdh-1 showed linkage with chl-1, the recombination value being 3.6% (Table 1). Pgi-1 is already known to be linked with chl-1 and v-1 (vires- cent-1) with recombination values of 27.2% and 34.4%, respectively (Ishikawa et al. 1991). Accordingly, the order of these genes was confirmed to be chl-1- Gdh-1-Pgi-1-v-1 as shown in Fig. 1.
Table 1. Linkage of Gdh-12 in Acc259 and chl-1 in FL58 found in the F2 population between FL58 and Acc259 ============================================================================== Gdh-1 Total F2 Recombination chl-1/+ ======================== plants value (%) 2/2 2/1 1/1 ============================================================================== (+/+ +/chl-1) 16 31 0 47 3.6+-2.43 chl-1/chl-1 0 2 11 13 ==============================================================================
Ishikawa, R., H. Morishima, T. Kinoshita, T. Harada, M. Niizeki and K. Saito, 1991. Linkage analysis of nine isozyme genes on the conventional linkage map in rice. Jpn. J. Breed. 41: 265-272.