Report of the Committee on Genetic Stocks (T. Omura) ............... 30

1. Establishment of a trisomic series in rice by using a desynaptic mutant, by H. Yasui, H. Satoh and N. Iwata ................ 50
2. Induction of X + 1 plants of rice by means of anther culture of trisomic plants, by Zi-Xuan Wang, Y. Oda and N. Iwata ............... 52
3. Karyotype analysis of Lu-Dao, an indigenous weedy rice germplasm found in Jiangsu, China, by Zeng-Jian Chen and Li-Hong Zhu ............... 54
4. Comparison of length between chromosomes 2 and 3, by H. I. Oka ............... 56

II. Interspecific crosses

5. Crossability of wild Oryza species and their potential use for improvement of cultivated rice, by L. A. Sitch, R. D. Dalmacio and G. O. Romero ............... 58
6. An Oryza sativa X O. officinalis hybrid obtained by using a male-sterile line as maternal parent, by Wen-Jin Yu ............... 60

III. Evolution and varietal differentiation

7. A supplementary note on the sickle-shaped rice cultivars, by K. S. Cheng, N. W. Huang and Y. Z. Zhang ............... 61
8. Genetic divergence among rices from Northeast India, by J. C. Glaszmann, P. Benyayer and M. Arnaud ............... 63
9. Two SDS-PAGE protein bands specific to subspecies indica and japonica of rice, by S. M. Zhang, C. Bai and L. S. Liu ............... 65
10. Plant opal analysis as a method of distinguishing between subspecies indica and japonica of O. sativa, by Y. I. Sato, H. Fujiwara, T. Udatsu and H. Morishima ............... 67
11. Distribution of gene diversity in Indica and Japonica rice varieties and their wild progenitors, by H. I. Oka ............... 70
12. Variation and inheritance of seed shedding in weedy rice, by Lin-Hua Tang and H. Morishima ............... 72
13. Additional observations and collection of the Oryza latifolia Desv. species complex, by G. Second ............... 73
14. Phylogenetic analysis of the tribe Oryzeae: Total chloroplast DNA restriction fragment analysis, by S. H. Zhang and G. Second ............... 76

IV. Segregation distortion

15. Abnornial segregation patterns in crosses between Oryza savita and O. glaberrima, by J. L. Pham and B. Bougerol ............... 80
16. Distorted F₂ ratios and changes in gene frequency observed for the wx and other three loci, by H. I. Oka ............... 83
17. Estimation of recombination values between loci C and wx from distorted F₂ segregation data, by H. I. Oka ............... 86
18. Comparative gametic selection in sexual and anther culture derivatives from five distant F₁, hybrids of rice, by E. Guiderdoni ............... 91

V. New genes and linkages

19. Gamete eliminator detected in the wild progenitor of Oryza sativa , by Y. Sano ............... 93
20. An intermediate amylose content determined by an allele at the wx locus, by M. Nishiniura and Y. Sano ............... 95
21. Gene Lk-f for long grain found in an induced mutant of variety Shiokari, by T. Kinoshita, H. Kikuchi and I. Takamure ............... 96
22. An induced dwarfing gene, sd-7(t), obtained in Taichung 65, by K. H. Tsai ............... 99
23. Allelism of genes for elongation of uppermost internode from two different sources, by M. Maekawa, T. Maekawa, N. Shinbashi and T. Kinoshita ............... 101
24. Identification of four new isozyme loci in rice, by B. G. delos Reyes, D. S. Brar and G. S. Khush ............... 103
25. Linkage map for Nishimura's chromosome 8, by N. Iwata, H. Satoh and A. Yoshimura ............... 106
26. Location of a mutant gene for rice storage protein esp-3 on Nishimura's chromosome 9, by T. Kumamaru, H. Satoh, T. Omura and M. Ogawa ............... 108
27. Linkage analysis for four isozyme loci, Adh-1, Acp-1, Pox-2 and Pgd- 2, by R. Ishikawa and H. Morishima ............... 109

VI. Physiological genetics

28. Genetics of leaf rolling under vegetative stage drought stress, by B. N. Singh and D. J. Mackill ............... 112
29. Genetic control of iron deficiency chlorosis tolerance in rice, by C. D. R. Reddy, Y. S. Nerkar and M. B. Misal ............... 115
30. A temperature-sensitive male-sterile line found in rice, by X. Z. Sun, S. K. Min and Z. M. Xiong ............... 116
31. Differential pollen tube elongation causing "alien pollen primacy" in rice, by Y. I. Sato ............... 117
32. Developmental changes in transcription activity of genes in rice seed, by X. H. Zhang and X. H. Tang ............... 119

VII. Genes for cold tolerance and disease resistance

33. Number of effective factors controlling cold tolerance at the anthesis stage, by M. Nishimura and K. Hamamura ............... 122
34. Location of a new gene for resistance to bacterial blight found in Yunnan local variety Haomei, by L. Y. Dai, D. P. Zhang and Y. F. Xie ............... 124

VIII. Cytoplasmic male sterility and related studies

35. Identifying potential sources of cytoplasmic male sterility in rice, by L. A. Sitch and R. D. Dalmacio ............... 125
36. Cytoplasmic diversity among CMS lines of rice, by S. S. Virmani, R. Vijaya Kumar and C. Casal ............... 127
37. Identification of wide compatibility genes in rice, by S. S. Virmani, R. Vijaya Kumar and P. S. Aurin ............... 129

IX. Mutation and gene action

38. Temperature sensitive chlorophyll mutations, by O. Yatou and X. Y. Cheng............... 131
39. Formation of abscission layer controlled by a shattering gene linked with semidwarfing gene sd-1 in rice, by S. Oba and F. Kikuchi ............... 132
40. Effects of dwarfing genes on proteins in rice grain observed in isogenic lines of variety Shiokari, by K. Watanabe and O. Kamijima ............... 134
41. Effects of dwarfing genes on coleoptile and mosocotyl elongation observed in isogenic lines of variety Shiokari, by K. Watanabe and O. Kamijima ............... 135
42. Protein kinase activity in diverse tissues of two isogenic lines of rice, by C. M. Cheng, K. H. Tsai and N. T. Hu ............... 137

43. Mutagenesis by means of anther culture combined with gamma irradiation, by T. Kinoshita, K. Mori and I. Takamure ............... 139
44. In vitro screening of the effects of antimitotics on differentiating rice tissues, by A. N. Sharma and U. Sinha ............... 141
45. 45. A female-sterile mutant found in a somaclonal line for IR50, by D. H. Ling, Z. R. Ma, M. F. Chen and W. Y. Chen ............... 143

46. The chloroplast-encoded ORF39 sequence of Oryza rufipogon, by Z. X. Luo, X. P. Zhao, J. C. Cote and Ray Wu ............... 144
47. Comparison of eleven chloroplast genes between an Indica and a Japonica cultivar of rice, by E. C. Kemmerer and Ray Wu ............... 147
48. Occasional biparental inheritance of chloroplast DNA in rice, by G. Second, A. Dally and S. H. Zhang ............... 150
49. RFLP analysis of rice mitochondrial DNA , by T. Ishii and K. Tsune- waki ............... 153
50. Heterogeneity of small plasmid-like DNAs found in rice mitochondria, by W. Sakamoto, K. Kadowaki, K. Harada, S. Tano and H. Yamaguchi ............... 155
51. Cloning and sequencing of mitochondrial encoded genes irt rice, by E. K. Kalelkau, C. Andre, B. Doshi and V. Walbot ............... 157
52. DNA structure of mutant genes: An observation of the wx locus, by O. Yatou, K. Kadowaki and E. Amano ............... 157
53. ransgenic rice plants produced from protoplasts following direct uptake of Bacillus thuringiensis δ-endotoxin protein gene, by H. Yang, S. D. Guo, J. X. L], X. J. Chen and Y. L. Fan ............... 159
54. Cloning and comparison of the atpA gene in mtDNA between male- sterile and normal rice lines, by D. D. Li and B. Wang ............... 161

55. Isolation of RNA and DNA from different rice tissues, by L. A. N. Hensgens and P. E. Van Os-Ruygrok ............... 163
56. Improved histochemical staining for β-D-glucuronidase activity in monocotyledonous plants, by S. Rueb and L. A. M. Hensgens ............... 168
57. Screening of totipotent rice cell line for efficient transformation, by A. P. Aryan and T. W. Okita ............... 170
58. High frequency of protoplast division and regeneration in Indica rice, by B. L. Sun, X.H. Li, Y.R. Sun, S.M. Zhao and J.Chen ............... 170
59. Plant regeneration from protoplasts of Indica and Japonica rices, by X. H. Li, A. Aryan, C. K. Xiang and T. Okita ............... 173
60. High regeneration frequency of protoplasts obtained in rice variety Zhonghua 8, by J. X. Li, H. Yang and Y. L. Fan ............... 175
61. A rapid, reliable method for preparing somatic chromosomes of rice, by N. Islam-Faridi and L. A. Sitch ............... 176