25. Mapping of genetic locus associated with resistance to brown planthopper in a line derived from the cross Oryza sativa x O. minuta
 
C. A. KUMAR1), S. SANTHANALAKSHMI1), D. K. SHIRALE2), P. SRINIVAS2), B. R. CHAR2), and P. KADIRVEL1,3)

1) Barwale Foundation, A.G. Heights, Road No. 12, Banjara Hills, Hyderabad India
2) Mahyco Research Centre, Jalna, Maharashtra, India
3) Corresponding author, E-mail: info@barwalefoundation.org, Tel: +91-40-23307632/23307812, Fax: +91-40-23325965

The brown planthopper (Nilaparvata lugens Stal) (BPH) is an important insect pest posing serious threat to rice production in India and other rice growing countries. Genetic resistance to BPH has been well documented in rice germplasm collection. Several major genes and quantitative trait loci (QTLs) have been reported for BPH resistance in varieties as well as wild species of rice (Khush and Brar, 1991 Soundararajan et al. 2004 Jena et al. 2005). In this study, an attempt was made to trace BPH resistance locus from IR71033-62-24, a derivative of Oryza sativa/O. minuta cross, using simple sequence repeat (SSR) markers.

A F3 segregating population consisting of 284 families was produced from the cross Mahsuri (BF- selection)/IR71033-62-24. The parents and F3 families were phenotyped for BPH resistance using standard seedbox screening test (SSST) as described by Heinrichs et al. (1985). BPH insects collected from the rice fields at Maruteru, Hyderabad and reared on the susceptible variety, TN1 were used for screening experiments. In greenhouse screening experiments, IR71033-62-24 showed moderate resistance (damage rating of 5) when Mahsuri (BF-selection) showed high level of susceptibility (damage rating of 9). The damage rating of F3 families ranged from 1 to 9 with an average of 6. Phenotypic segregation in F3 families did not conform to Mendelian segregation ratios indicating the quantitative nature of resistance in this population (Fig. 1). There was a strong indication of transgressive variation for BPH resistance in this population. Out of 284 F3 families analyzed, 87 showed higher level of resistance (damage rating of less than 5) compared to the resistant parent IR71033-62-24.

An attempt was made to detect genetic locus or loci contributing to BPH resistance in IR71033-62- 24 through bulked segregant analysis (BSA) using SSR markers. The results indicated that out of 125 polymorphic SSRs, six markers: RM585, RM225 and RM204 on chromosome 6, RM10 and RM346 on chromosome 7 and RM264 on chromosome 8 showed polymorphism between resistant and susceptible bulks as well as individuals constituting the bulks. A subset of 75 F2 individuals was genotyped with the positive markers identified in BSA for marker-trait association. Single locus analysis through one-way ANOVA showed that SSR marker RM585 on chromosome 6 had significant association with BPH resistance (P=0.000) (Table 1). The mean phenotypic values of F3 families carrying marker alleles (homozygous) of IR71033-62-24 was lower than F3 families carrying marker alleles (homozygous) of Mahsuri (BF-selection) for the putative marker associated with BPH resistance. These results suggested the presence of a putative genetic locus for BPH resistance on chromosome 6 (near the marker RM585). The location of the putative BPH resistance locus detected in the mapping population is shown in the SSR linkage map published by Temnyk et al. (2000) (Fig. 2). The region on chromosome 6 carrying this putative locus is also reported to harbor a major gene bph4 as well as a QTL (Alam and Cohen, 1998 Kawaguchi et al. 2001 Soundararajan et al. 2004) however, the allelic relationship remains to be established.

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References
Heinrichs E. A., F. G. Medrano and H. R. Rapusas, 1985. Genetic evaluation for insect resistance in rice. International Rice Research Institute, Manila, Philippines, 356 pp.

Jena K. K., J. U. Jeung, J. H. Lee, H. C. Choi and D. S. Brar, 2006. High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice Oryza sativa L.). Theor. Appl. Genet. 112: 288-297.

Kawaguchi M., K. Murata, T. Ishi, S. Takumi, N. Mori and C. Nakamura, 2001. Assignment of a brown planthopper (Nilaparavata lugens Stål) resistance gene bph4 to the rice chromosome 6. Breed. Sci. 51: 13-18.

Khush G. S. and D. S. Brar, 1991. Genetics of resistance to insects in crop plants. Adv. Agron. 45: 223- 274.

Soundararajan R. P., P. Kadirvel, K. Gunathilagaraj and M. Maheswaran, 2004. Mapping of quantitative trait loci associated with resistance to brown planthopper in rice by means of a doubled haploid population. Crop Sci. 44: 2214-2220.

Temnykh S., W. D. Park, N. Ayres, S. Cartinhour, S. N. Hauck, L. Lipovich, Y. G. Cho, T. Ishii and S. R. McCouch, 2000. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100: 697-712.



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