16. Identification of a dominant suppressor of photoperiod-sensitive gene using indica/japonica   backcrossed progenies in rice (Oryza sativa L.)

C. Y. YU 1,2, X. J. WEI 1, L. M. CHEN1, L. JIANG 1, H. Q. ZHAI3 and J. M. WAN1,3

1) State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095 China

2) .Jiangxi Academy of Agricultural Sciences, Nanchang, 330200 China

3) Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081 China

Correspondence should be addressed to Wan Jianmin (E-mail:wanjm@njau.edu.cn)


Photoperiod-sensitivity is one of the most important features of rice adaptability. There are two major types of photoperiod-sensitive genes, Se and E, in rice (Okumoto et al. 1996, Ichitani et al. 1998). Meanwhile, some modification factors such as i-Se-1 and Su-Se-1 (t) suppressed the expression of Se1 gene (Chang et al. 1969, Sano 1990, Ohshima et al. 1993). We observed that IR24 was a photoperiod-insensitive indica and Asominori was a photoperiod-sensitive japonica cultivar. Under long-day growing environments, the days to heading (hereafter abbreviated as DTH) of IR24×Asominori F1 exhibited great over-parental heterosis and the DTH of the RIL population derived from IR24×Asominori (Tsunematsu et al. 1995) showed apparently transgressive segregation, which suggested that photoperiod-sensitive genes and their suppression factors co-existed in IR24.

To identify photoperiod-sensitive suppression genes, we constructed two F1 populations using a CSSL population derived from Asominori×IR24 with genetic background of Asominori genome (Kubo et al. 1998) as female parents and Asominori and IR24 as pollen parents. We planted the two F1 populations and all CSSLs under natural long-day environment (Nanjing, 32o 04'N) to observe the phenotypic data of DTH. In the winter of 2004, we also planted the CSSL population in Hainan (18o 29'N). In this study, we defined the DTH difference of the same line between Nanjing and Hainan as photoperiod-sensitive effect of DTH. The results showed that: 1) the CSSLs (hereafter we temporarily called these CSSLs as CSSLs-6) containing the substituted segment of chromosome 6 linked with RFLP marker R2171 (Fig. 1) had average DTH 7 days shorter than that of Asominori (Fig. 2A), 2) CSSLs-6 had no photoperiod-sensitivity (Fig. 2B), which indicated that Asominori had lost photoperiod-sensitivity due to the introgression of the allele of IR24, 3) The combinations of CSSLs-6 with Asominori had DTH as early as that of the early maturing CSSLs-6 (Fig. 2A) and the F1s with IR24 had great negative heterosis for DTH (Fig. 2C), which indicated that the allele of IR24 was a dominant photoperiod-sensitive suppression gene. We tentatively designated this gene as Su-PS-6(t).

 To further understand the photoperiod-sensitive suppression effect of Su-PS-6(t), we created some hybrids of Asominori, IR24 and AIS43, one of CSSLs-6, crossing with two indica and three japonica cultivars, and planted all the hybrids at Nanjing in the summer of 2005. The DTH of all the hybrids of AIS43 exhibited early while the inter-subspecific hybrids of Asominori performed over-parental late maturing and the hybrids of IR24 had no DTH heterosis (Table 1), which further verified that Su-PS-6(t) was a dominant photoperiod-sensitive suppression gene. When Su-PS-6(t) was introduced into restorer or sterile lines,  it could suppress the expression of some photoperiod-sensitive genes. Thus, Su-PS-6(t) eliminated the interaction effects between photoperiod-sensitive genes from both parents of hybrids and significantly promoted heading of the intra- or inter-subspecific hybrids. Whether Su-PS-6(t) identified here is the same as Su-Se-1(t), a dominant suppressor of photoperiod-sensitive gene Se1 documented early (Ohshima et al. 1993, Sano 1990) or not, it is need to conduct further experiments.


Table 1. DTH of testers and the combinations between AIS43, Asominori, IR24

and testers in the summer of 2005, Nanjing, China


DTH of tester

DTH of combination (days)





R084 (indica)





9311 (indica)





C418 (japonica)





02428 (japonica)





Balila (japonica)





















Fig.1. Graphical genotype analyses of CSSLs containingSu-PS-6 (t) on chr. 6. The black horizontal bars represent chromosome substituted segments.
Fig. 2. Analyses of photoperiod-sensitive suppression effect for Su-PS-6(t). 1, 2, 3, 4, 5 and 6 on X-axis represent CSSL AIS11, AIS43, AIS 44, AIS 45, AIS47 and Asominori, respectively. A: DTH of CSSL (left white bar) and corresponding CSSL/Asominori F1 (right bar). B: Photoperiod-sensitive effect of DTH. C: Mid-parent heterosis of CSSLs/IR24.


Chang, T. T., C. C. Li and B. S. Vergara, 1969. Component analysis of duration from seeding to heading in rice by the basic vegetative phase and photoperiod-sensitive phase. Euphytica. 18: 79-91.

Okumoto, Y., H. Inoue, K. Ichitani and T. Tanisaka, 1996. Effects of three genes that control heading date (Se1, E1 and Ef1) in four varietal groups of Japonica rice [A]. In: Rice Genetics III [C]. IRRI, Manila Philippines. 423-427.

Ichitani, K., Y. Okumoto and T. Tanisaka, 1998. Genetic analysis of the rice cultivar Kasalath with special reference to two photoperiod sensitivity loci E1 and Se-1. Breed. Science. 48: 51-57.

Tsunematsu, H, A. Yoshimura, Y. Harushima, Y. Nagamura, N. Kurata, M. Yano, T. Sasaki and N. Iwata, 1996. RFLP framework map using recombinant inbred lines in rice. Breed. Science. 46: 279-284.

Kubo, T., K. Nakamura and A. Yoshimura, 1999. Development of a series of Indica chromosome segment substitution lines in Japonica background of rice. RGN 19: 104-106.

Ohshima, I., Y. Watanabe and C. Asahi, 1993. Genetic analysis of heading time in cross between two Indica varieties with two inhibitor genes for photoperiod sensitivity. Jpan. J. Breed. 43: 101-106.

Sano, Y., 1990. A dominant suppressor for the photoperiod sensitive gene, Se-1, detected in a photoperiod-sensitive cultivar of an Indica type. RGN 7: 101-103.